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12 results for " Growth Factors Hormones ELISA Kits" - showing 1-12


Immunohistochemistry (IHC) (Figure 2. IHC analysis of EGF using anti-EGF antibody (MBS1750382). EGF was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EGF Antibody (MBS1750382) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

EGF, Polyclonal Antibody (Cat# AAA1750382)

Full Name
Anti-EGF Picoband Antibody
Reactivity
Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of IGFBP1 using anti-IGFBP1 antibody (MBS1750517).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant mouse IGFBP1 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP1 at approximately 34KD. The expected band size for IGFBP1 is at 27KD. )

IGFBP1, Polyclonal Antibody (Cat# AAA1750517)

Full Name
Anti-IGFBP1 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data (Standard Curve for Human EGF: Capture Antibody Mouse mAb 201165-5E9 (5E9-A10-A6) to Human EGF at 4mug/ml and Detector Antibody Mouse mAb 201165-7C11 (7C11-G10-H8) to Human EGF at 1mug/ml.)

EGF, Monoclonal Antibody (Cat# AAA475545)

Full Name
Anti-Human EGF Mouse mAb
Gene Names
EGF; URG; HOMG4
Reactivity
Human
Applications
EIA
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)

FGF7/KGF, ELISA Kit (Cat# AAA1751077)

Full Name
Monkey primate FGF7/KGF PicoKine ELISA Kit
Gene Names
FGF7; KGF; HBGF-7
Reactivity
Monkey
There is no detectable cross-reactivity with other relevant proteins.
Applications
EIA
Pricing
$460/96-Strip-Wells | $2,060/5x96-Strip-Wells
Testing Data (Standard Curve for Human EGF: Capture Antibody Mouse mAb 201165-5E9 (5E9-A10-A6) to Human EGF at 4mug/ml and Detector Antibody Mouse mAb 201165-7C11 (7C11-G10-H8) to Human EGF at 1mug/ml.)

EGF, Monoclonal Antibody (Cat# AAA475295)

Full Name
Anti-Human EGF Mouse mAb
Gene Names
EGF; URG; HOMG4
Reactivity
Human
Applications
EIA
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of NAP2 using anti-NAP2 antibody (MBS1750787).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse bone tissue lysates,Lane 2: mouse lung tissue lysates,Lane 3: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAP2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NAP2 at approximately 11KD. The expected band size for NAP2 is at 14KD. )

NAP2, Polyclonal Antibody (Cat# AAA1750787)

Full Name
Anti-NAP2 Picoband antibody
Gene Names
Ppbp; TGB; LDGF; MDGF; TGB1; CTAP3; Cxcl7; NAP-2; Scyb7; b-TG1; LA-PF4; THBGB1; CTAPIII; beta-TG; AI854500; NAP-2-L1; 2400003M24Rik
Reactivity
Mouse
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of EGF expression in human urine sample lysate (MBS179296).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGF monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EGF )

EGF, Monoclonal Antibody (Cat# AAA179296)

Full Name
Anti-EGF Rabbit Monoclonal Antibody
Gene Names
EGF; URG; HOMG4
Reactivity
Human
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of PGF using anti-PGF antibody (MBS1750586).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat gaster tissue lysates,Lane 2: rat liver tissue lysates,Lane 3: mouse gaster tissue lysates,Lane 4: mouse liver tissue lysates,Lane 5: mouse HEPA1-6 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGF antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PGF at approximately 25KD. The expected band size for PGF is at 25KD. )

PGF/Plgf, Polyclonal Antibody (Cat# AAA1750586)

Full Name
Anti-PGF/Plgf Picoband Antibody
Gene Names
Pgf; Plgf
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of KGF using anti-KGF antibody (MBS1750731).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse brain tissue lysates,Lane 2: rat RH35 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KGF antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KGF at approximately 22KD. The expected band size for KGF is at 22KD. )

KGF, Polyclonal Antibody (Cat# AAA1750731)

Full Name
Anti-KGF Picoband Antibody
Gene Names
Fgf7; Kgf
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis of IP10 expression in IP10 recombinant protein lysate (MBS179236).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL10 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CXCL10 )

IP10, Monoclonal Antibody (Cat# AAA179236)

Full Name
Anti-IP10 Rabbit Monoclonal Antibody
Gene Names
CXCL10; C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)

TNFSF13/APRIL, ELISA Kit (Cat# AAA1751081)

Full Name
Monkey primate TNFSF13/APRIL PicoKine ELISA Kit
Reactivity
Monkey
There is no detectable cross-reactivity with other relevant proteins.
Applications
EIA
Pricing
$460/96-Strip-Wells | $2,060/5x96-Strip-Wells
Western Blot (WB) (Figure 1. Western blot analysis of IGFBP1 using anti-IGFBP1 antibody (MBS1750516).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP1 at approximately 30KD. The expected band size for IGFBP1 is at 30KD. )

IGFBP1, Polyclonal Antibody (Cat# AAA1750516)

Full Name
Anti-IGFBP1 Picoband Antibody
Gene Names
IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
Reactivity
Human
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
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