Succinate Dehydrogenase Complex Subunit C Recombinant Protein | SDHC recombinant protein

Recombinant Succinate Dehydrogenase Complex Subunit C (SDHC)

Cat. Num. AAA2033144

• Gene Names: SDHC; CYBL; PGL3; QPS1; SDH3; CYB560
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Succinate Dehydrogenase Complex Subunit C; Recombinant Succinate Dehydrogenase Complex Subunit C (SDHC); PGL3; CYB560; SDH3; CYBL; QPs1; Integral membrane protein CII-3; Succinate-ubiquinone oxidoreductase cytochrome B large subunit; Succinate dehydrogenase cytochrome b560; SDHC recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MAAFLLRHVS RHCLRAHLNA QLCIRNAAPL GTTAKEEMER FWKKNTSSNRPLSPHLTIYK WSLPMALSVC HR
• Sequence Length: 135

• Applicable Applications for SDHC recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 38.3kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Arg72
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and GST-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 78096639
• NCBI GeneID: 6391
• Molecular Weight: 12,474 Da
• NCBI Official Full Name: succinate dehydrogenase complex, subunit C delta3 alternative splicing variant
• NCBI Official Synonym Full Names: succinate dehydrogenase complex subunit C
• NCBI Official Symbol: SDHC
• NCBI Official Synonym Symbols: CYBL; PGL3; QPS1; SDH3; CYB560
• NCBI Protein Information: succinate dehydrogenase cytochrome b560 subunit, mitochondrial
• UniProt Protein Name: Succinate dehydrogenase cytochrome b560 subunit, mitochondrial
• Protein Family: Succinate dehydrogenase 2 membrane
• UniProt Gene Name: SDHC
• UniProt Synonym Gene Names: CYB560; SDH3; QPs1; CYBL
• UniProt Entry Name: C560_HUMAN

NCBI Description

This gene encodes one of four nuclear-encoded subunits that comprise succinate dehydrogenase, also known as mitochondrial complex II, a key enzyme complex of the tricarboxylic acid cycle and aerobic respiratory chains of mitochondria. The encoded protein is one of two integral membrane proteins that anchor other subunits of the complex, which form the catalytic core, to the inner mitochondrial membrane. There are several related pseudogenes for this gene on different chromosomes. Mutations in this gene have been associated with paragangliomas. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2013]

Uniprot Description

SDHC: Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHC are the cause of paragangliomas type 3 (PGL3). A neural crest tumor usually derived from the chromoreceptor tissue of a paraganglion. Paragangliomas are most commonly located in the head and neck region, specifically at the carotid bifurcation, the jugular foramen, the vagal nerve, and in the middle ear. Defects in SDHC are a cause of paraganglioma and gastric stromal sarcoma (PGGSS); also known as Carney- Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Belongs to the cytochrome b560 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Energy Metabolism - oxidative phosphorylation; Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrial respiratory chain complex II; mitochondrion

Molecular Function: electron carrier activity; heme binding; metal ion binding; succinate dehydrogenase activity

Biological Process: aerobic respiration; cellular metabolic process; mitochondrial electron transport, succinate to ubiquinone; tricarboxylic acid cycle

Disease: Gastrointestinal Stromal Tumor; Paraganglioma And Gastric Stromal Sarcoma; Paragangliomas 3