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SDS-Page

Syndecan 4 Recombinant Protein | SDC4 recombinant protein

Recombinant Syndecan 4 (SDC4)

Gene Names
SDC4; SYND4
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Syndecan 4; Recombinant Syndecan 4 (SDC4); SDC4 recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
4.5 (varies by lot)
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-AES IRETEVIDPQ DLLEGRYFSG ALPDDEDVVG PGQESDDFEL SGSGDLDDLE DSMIGPEVVH PLVPLDNHIP ERAGSGSQVP TEPKKLEENE VIPKRISPVE ESEDVSNKVS MSSTVQGSNI FERTE
Sequence Length
198
Applicable Applications for SDC4 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
17.8kDa
Accurate Molecular Mass (KD)
24kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Homo sapiens (Human)
Fragment
Ala18~Glu145
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
16,545 Da
NCBI Official Full Name
Syndecan 4
NCBI Official Synonym Full Names
syndecan 4
NCBI Official Symbol
SDC4
NCBI Official Synonym Symbols
SYND4
NCBI Protein Information
syndecan-4
UniProt Protein Name
Syndecan-4
Protein Family
UniProt Gene Name
SDC4
UniProt Synonym Gene Names
SYND4
UniProt Entry Name
SDC4_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-4: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Expressed as a homodimer. Promotes cell spreading in a beta(1) integrin-dependent fashion through PKC-alpha and Rho.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: lysosomal lumen; focal adhesion; costamere; cell surface; integral to plasma membrane; Golgi lumen; plasma membrane; lipid raft

Molecular Function: protein binding; protein kinase C binding; fibronectin binding

Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; phototransduction, visible light; glycosaminoglycan catabolic process; extracellular matrix organization and biogenesis; positive regulation of focal adhesion formation; glycosaminoglycan metabolic process; ureteric bud development; positive regulation of protein kinase activity; carbohydrate metabolic process; positive regulation of stress fiber formation; pathogenesis; retinoid metabolic process

Research Articles on SDC4

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Product Notes

The SDC4 sdc4 (Catalog #AAA2029621) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Syndecan 4 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the SDC4 sdc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF-AES IRETEVIDPQ DLLEGRYFSG ALPDDEDVVG PGQESDDFEL SGSGDLDDLE DSMIGPEVVH PLVPLDNHIP ERAGSGSQVP TEPKKLEENE VIPKRISPVE ESEDVSNKVS MSSTVQGSNI FERTE
. It is sometimes possible for the material contained within the vial of "Syndecan 4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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