CD138 recombinant protein

CD138 Recombinant Protein

Cat. Num. AAA3003914

• Gene Names: SDC1; SDC; CD138; SYND1; syndecan
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Synonyms: CD138; CD138 Recombinant Protein; CD138 recombinant protein

• Host: E Coli
• Purity/Purification: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Form/Format: PBS, 4M Urea, PH7.4
• Concentration: >=0.5mg/ml (varies by lot)
• Sequence: QIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLG

• Tag: His-tag in C-terminal
• Expression Vector: pet-22b(+)
• BP: 708
• Restriction Site: NdeI-XhoI
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Testing Data

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Related Product Information for CD138 recombinant protein

Background: Syndecans are a family of type 1 transmembrane heparan sulphate proteoglycans comprising four members in mammals (SDC-1 to -4) encoded by four syndecan genes. Syndecans are involved in embryonic development, tumorigenesis, and angiogenesis. The extracellular domain harbors attachment sites for heparan sulfate and chondroitin sulfate chains, facilitating interaction with an array of proteins including a plethora of growth factors. In addition, the hydrophobic C-terminal intracellular domain can interact with proteins containing a PDZ domain. These interactions place syndecans as important integrators of membrane signaling. Syndecans undergo proteolytic cleavage causing the release of their extracellular domain (shedding), converting the membrane-bound proteins into soluble molecular effectors. Syndecan 1 (SDC1) is a specific marker for plasmacytic differentiation in hematologic disorders. This cell surface proteoglycan is also expressed in normal epithelial cells and tissues as well as various types of cancer tissues. The extracellular shed form of syndecan 1 remains soluble or accumulates in the extracellular matrix where it binds growth factors, cytokines and other extracellular matrix proteins. This binding activates signaling of bound growth factors or cytokines, which results in enhanced tumor growth, dissemination, angiogenesis, and osteolysis. As a result, the level of syndecan1 protein and its shed form may serve as prognostic factors for a list of malignancies.

NCBI and Uniprot Product Information

• NCBI GI #: 55749480
• NCBI GeneID: 6382
• NCBI Accession #: NP_001006947.1
• NCBI GenBank Nucleotide #: NM_001006946.1
• UniProt Accession #: P18827; Q53QV0; Q546D3; Q96HB7; D6W523; AAB21434; AAB23935
• Molecular Weight: 32,462 Da
• NCBI Official Full Name: syndecan-1
• NCBI Official Synonym Full Names: syndecan 1
• NCBI Official Symbol: SDC1
• NCBI Official Synonym Symbols: SDC; CD138; SYND1; syndecan
• NCBI Protein Information: syndecan-1; CD138 antigen; syndecan proteoglycan 1; heparan sulfate proteoglycan fibroblast growth factor receptor
• UniProt Protein Name: Syndecan-1
• UniProt Gene Name: SDC1
• UniProt Synonym Gene Names: SDC; SYND1
• UniProt Entry Name: SDC1_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family.

Protein type: Motility/polarity/chemotaxis; Extracellular matrix; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: lysosomal lumen; focal adhesion; cell surface; protein complex; Golgi lumen; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein C-terminus binding; protein binding; glycoprotein binding

Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; response to cAMP; myoblast development; wound healing; glycosaminoglycan metabolic process; striated muscle cell development; response to toxin; response to glucocorticoid stimulus; pathogenesis; lipoprotein metabolic process; Wnt receptor signaling pathway through beta-catenin; Sertoli cell development; odontogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; response to hydrogen peroxide; ureteric bud development; carbohydrate metabolic process; inflammatory response; response to calcium ion; retinoid metabolic process