Rabbit anti-Human SCO2 Polyclonal Antibody | anti-SCO2 antibody

Rabbit Polyclonal to Human SCO2

Cat. Num. AAA240375

• Gene Names: SCO2; MYP6; SCO1L; CEMCOX1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: SCO2; Polyclonal Antibody; Rabbit Polyclonal to Human SCO2; Anti-SCO2 Antibody (C-Terminus) IHC-plus; SCO1L; Human SCO2; anti-SCO2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: 19 amino acid peptide from near the carboxy terminus of human SCO2.
• Purity/Purification: Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 266

• Applicable Applications for anti-SCO2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (2.5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human SCO2.
• Immunogen Type: Synthetic peptide
• Immunogen: SCO2 antibody was raised against synthetic peptide from human SCO2.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-SCO2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SCO2 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of SCO2 in Human Liver cells with SCO2 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of SCO2 in human liver tissue lysate with SCO2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.)

Related Product Information for anti-SCO2 antibody

Mammalian cytochrome c oxidase (COX) catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. In yeast, 2 related COX assembly genes, SCO1 and SCO2 (synthesis of cytochrome c oxidase), enable subunits 1 and 2 to be incorporated into the holoprotein. This gene is the human homolog of the yeast SCO2 gene.

NCBI and Uniprot Product Information

• NCBI GI #: 153791313
• NCBI GeneID: 9997
• NCBI Accession #: NP_005129.2
• NCBI GenBank Nucleotide #: NM_005138.2
• UniProt Accession #: O43819; Q3T1B5; Q9UK87
• Molecular Weight: 29,810 Da
• NCBI Official Full Name: protein SCO2 homolog, mitochondrial
• NCBI Official Synonym Full Names: SCO2 cytochrome c oxidase assembly protein
• NCBI Official Symbol: SCO2
• NCBI Official Synonym Symbols: MYP6; SCO1L; CEMCOX1
• NCBI Protein Information: protein SCO2 homolog, mitochondrial; SCO cytochrome oxidase deficient homolog 2
• UniProt Protein Name: Protein SCO2 homolog, mitochondrial
• Protein Family: SCO2-like protein
• UniProt Gene Name: SCO2
• UniProt Entry Name: SCO2_HUMAN

NCBI Description

Cytochrome c oxidase (COX) catalyzes the transfer of electrons from cytochrome c to molecular oxygen, which helps to maintain the proton gradient across the inner mitochondrial membrane that is necessary for aerobic ATP production. Human COX is a multimeric protein complex that requires several assembly factors; this gene encodes one of the COX assembly factors. The encoded protein is a metallochaperone that is involved in the biogenesis of cytochrome c oxidase subunit II. Mutations in this gene are associated with fatal infantile encephalocardiomyopathy and myopia 6. [provided by RefSeq, Oct 2014]

Uniprot Description

SCO2: Acts as a copper chaperone, transporting copper to the Cu(A) site on the cytochrome c oxidase subunit II (COX2). Defects in SCO2 are the cause of fatal infantile cardioencephalomyopathy with cytochrome c oxidase deficiency (FIC). This disease is characterized by hypertrophic cardiomyopathy, lactic acidosis, and gliosis. Heart and skeletal muscle show reductions in cytochrome c oxidase (COX) activity, whereas liver and fibroblasts show mild COX deficiencies. Belongs to the SCO1/2 family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: nucleoplasm; myofibril; mitochondrion; mitochondrial inner membrane

Molecular Function: copper ion binding

Biological Process: cellular copper ion homeostasis; eye development; in utero embryonic development; respiratory chain complex IV assembly; response to activity; copper ion transport; muscle system process

Disease: Cardioencephalomyopathy, Fatal Infantile, Due To Cytochrome C Oxidase Deficiency 1; Myopia 6