Human Serum amyloid A-1 protein ELISA Kit | SAA1 elisa kit

Human Serum amyloid A-1 protein ELISA Kit

Cat. Num. AAA2884408

• Gene Names: SAA1; SAA; PIG4; SAA2; TP53I4
• Reactivity: Human
• Synonyms: Serum amyloid A-1 protein; Human Serum amyloid A-1 protein ELISA Kit; SAA; SAA1; SAA1 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human SAA
• Sequence Length: 122

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 3.12-200 ng/mL
• Sensitivity: < 1.56 ng/mL
• Intra-assay Precision: <=3.1%
• Inter-assay Precision: <=6.2%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for SAA1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 40316912
• NCBI GeneID: 6288
• NCBI Accession #: NP_000322.2
• NCBI GenBank Nucleotide #: NM_000331.5
• UniProt Accession #: P0DJI8; P02735; P02736; P02737; Q16730; Q16834; Q16835; Q16879; Q3KRB3; Q6FG67; Q96QN0; Q9UCK9
• Molecular Weight: 13,532 Da
• NCBI Official Full Name: serum amyloid A-1 protein preproprotein
• NCBI Official Synonym Full Names: serum amyloid A1
• NCBI Official Symbol: SAA1
• NCBI Official Synonym Symbols: SAA; PIG4; SAA2; TP53I4
• NCBI Protein Information: serum amyloid A-1 protein
• UniProt Protein Name: Serum amyloid A-1 protein
• Protein Family: Serum amyloid A-1 protein
• UniProt Gene Name: SAA1
• UniProt Synonym Gene Names: SAA
• UniProt Entry Name: SAA1_HUMAN

NCBI Description

This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11. [provided by RefSeq, Feb 2016]

Uniprot Description

SAA1: Major acute phase reactant. Apolipoprotein of the HDL complex. Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Elevated serum SAA1 protein levels may be associated with lung cancer. Belongs to the SAA family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytoplasmic microtubule; cytosol; extracellular region; extracellular space

Molecular Function: chemoattractant activity; G-protein-coupled receptor binding; heparin binding

Biological Process: activation of MAPK activity; acute-phase response; cellular protein metabolic process; elevation of cytosolic calcium ion concentration; innate immune response; lymphocyte chemotaxis; macrophage chemotaxis; negative regulation of inflammatory response; neutrophil chemotaxis; platelet activation; positive chemotaxis; positive regulation of cell adhesion; positive regulation of cytokine secretion; positive regulation of interleukin-1 secretion; receptor-mediated endocytosis; regulation of protein secretion