Mouse Protein S100-B ELISA Kit | S100b elisa kit

Mouse Protein S100-B ELISA Kit

Cat. Num. AAA2883366

• Gene Names: S100b; Bpb; AI850290
• Reactivity: Mouse
• Synonyms: Protein S100-B; Mouse Protein S100-B ELISA Kit; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100b; S100b elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Rat Follitropin subunit beta
• Sequence Length: 92

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids
• Detection Range: 0.156-10 mIU/mL
• Intra-assay Precision: Intra-Assay CV: <=5.9%
• Inter-assay Precision: Inter-Assay CV: <=8.1%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100b elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6677839
• NCBI GeneID: 20203
• NCBI Accession #: NP_033141.1
• NCBI GenBank Nucleotide #: NM_009115.3
• UniProt Accession #: P50114
• Molecular Weight: 10,728 Da
• NCBI Official Full Name: protein S100-B
• NCBI Official Synonym Full Names: S100 protein, beta polypeptide, neural
• NCBI Official Symbol: S100b
• NCBI Official Synonym Symbols: Bpb; AI850290
• NCBI Protein Information: protein S100-B
• UniProt Protein Name: Protein S100-B
• Protein Family: Protein
• UniProt Gene Name: S100b
• UniProt Entry Name: S100B_MOUSE

Uniprot Description

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family.

Protein type: Calcium-binding

Cellular Component: cell soma; cytoplasm; extracellular region; extracellular space; intracellular membrane-bound organelle; nucleus; perinuclear region of cytoplasm; ruffle

Molecular Function: calcium ion binding; calcium-dependent protein binding; identical protein binding; metal ion binding; protein homodimerization activity; RAGE receptor binding; receptor binding; zinc ion binding

Biological Process: cellular calcium ion homeostasis; energy reserve metabolic process; learning and/or memory; memory; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of synaptic transmission; regulation of cell shape; regulation of cytokine biosynthetic process; regulation of long-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity