S100-A9 recombinant protein

Recombinant Rat Protein S100-A9 (S100a9)

Cat. Num. AAA9423630

• Gene Names: S100a9; Mrp14
• Reactivity: Rat
• Purity: Greater than 80% as determined by SDS-PAGE.
• Synonyms: S100-A9; Recombinant Rat Protein S100-A9 (S100a9); Calgranulin-B; S100a9; S100-A9 recombinant protein

• Host: E Coli
• Reactivity: Rat
• Purity/Purification: Greater than 80% as determined by SDS-PAGE.
• Form/Format: Tris-based buffer, 50% glycerol
• Sequence Positions: Full Length, 2-113aa

• Tag Info: N-terminal 6xHis-SUMO-tagged
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.

Related Product Information for S100-A9 recombinant protein

S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and Extracellular domain functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The Extracellular domain functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, NXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 16758364
• NCBI GeneID: 94195
• NCBI Accession #: NP_446039.1
• NCBI GenBank Nucleotide #: NM_053587.1
• UniProt Accession #: P50116; Q761U7
• Molecular Weight: 28.32 kDa
• NCBI Official Full Name: protein S100-A9
• NCBI Official Synonym Full Names: S100 calcium binding protein A9
• NCBI Official Symbol: S100a9
• NCBI Official Synonym Symbols: Mrp14
• NCBI Protein Information: protein S100-A9
• UniProt Protein Name: Protein S100-A9
• UniProt Gene Name: S100a9
• UniProt Synonym Gene Names: Mrp14; MRP-14; p14

NCBI Description

calcium binding protein that may be associated with acute inflammatory processes [RGD, Feb 2006]

Uniprot Description

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: cell junction; cytoplasm; cytosol; extracellular matrix; extracellular space; nucleoplasm; nucleus

Biological Process: actin cytoskeleton reorganization; astrocyte development; autophagy; caspase activation; chronic inflammatory response; leukocyte chemotaxis; leukocyte migration during inflammatory response; neutrophil chemotaxis; peptidyl-cysteine S-nitrosylation; positive regulation of blood coagulation; positive regulation of inflammatory response; positive regulation of peptide secretion; regulation of integrin biosynthetic process; regulation of translation; response to ethanol; response to lipopolysaccharide; response to zinc ion