Rabbit mGluR-6 Polyclonal Antibody | anti-mGluR-6 antibody

mGluR-6 (A865) Polyclonal Antibody

Cat. Num. AAA3000554

• Gene Names: GRM6; mGlu6; CSNB1B; GPRC1F; MGLUR6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: mGluR-6; Polyclonal Antibody; mGluR-6 (A865) Polyclonal Antibody; Metabotropic glutamate receptor 6; mGluR6; GRM6; GPRC1F; MGLUR6; anti-mGluR-6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: mGluR6 (A865) polyclonal antibody detects endogenous levels of mGluR6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 877

• Applicable Applications for anti-mGluR-6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 841-890 of Human mGluR-6.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of mGluR-6 (A865) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:NIH-3T3 cell lysateLane3:Raw264. 7 cell lysateLane4:H9C2 cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of mGluR-6 (A865) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-mGluR-6 antibody

The mGluR proteins (metabotropic glutamate receptors) are members of the G protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic glutamate receptors). The eight currently known mGluR proteins are mediated by two G proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR-1 and mGluR-5 are mediated by a G protein that activates a phosphatidylinositolcalcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight subtypes of mGluR have an activity mediated by a G protein that inhibits adenylate cyclase activity. mGluR-6 is expressed in the synapses of bipolar cell dendrites. This receptor is involved in mediating synaptic transmission from rod and cone photoreceptors to other neurons.

NCBI and Uniprot Product Information

• NCBI GI #: 110611176
• NCBI GeneID: 2916
• NCBI Accession #: NP_000834.2
• NCBI GenBank Nucleotide #: NP_000834.2
• UniProt Accession #: O15303
• Molecular Weight: ~ 100kDa
• NCBI Official Full Name: metabotropic glutamate receptor 6
• NCBI Official Synonym Full Names: glutamate metabotropic receptor 6
• NCBI Official Symbol: GRM6
• NCBI Official Synonym Symbols: mGlu6; CSNB1B; GPRC1F; MGLUR6
• NCBI Protein Information: metabotropic glutamate receptor 6
• UniProt Protein Name: Metabotropic glutamate receptor 6
• UniProt Gene Name: GRM6
• UniProt Synonym Gene Names: GPRC1F; MGLUR6; mGluR6
• UniProt Entry Name: GRM6_HUMAN

NCBI Description

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Mutations in this gene result in congenital stationary night blindness type 1B. [provided by RefSeq, May 2018]

Uniprot Description

mGluR6: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. Defects in GRM6 are the cause of congenital stationary night blindness type 1B (CSNB1B). This disorder consits of a previously unrecognized, autosomal recessive form of congenital night blindness associated with a negative electroretinogram waveform. Patients are night blind from an early age, and when maximally dark-adapted, they could perceive lights only with an intensity equal to or slightly dimmer than that normally detected by the cone system. ERGs in response to single brief flashes of light have clearly detectable a-waves, which are derived from photoreceptors, and greatly reduced b-waves, which are derived from the second-order inner retinal neurons. ERGs in response to sawtooth flickering light indicate a markedly reduced ON response and a nearly normal OFF response. There is no subjective delay in the perception of suddenly appearing white vs black objects on a gray background. Belongs to the G-protein coupled receptor 3 family.

Protein type: Receptor, GPCR; GPCR, family 3; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: presynaptic membrane; Golgi membrane; endoplasmic reticulum membrane; integral to plasma membrane; dendrite; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; group III metabotropic glutamate receptor activity; protein homodimerization activity; glutamate receptor activity

Biological Process: synaptic transmission; regulation of synaptic transmission, glutamatergic; retina development in camera-type eye; metabotropic glutamate receptor signaling pathway; detection of light stimulus involved in visual perception; locomotory behavior; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway; detection of visible light

Disease: Night Blindness, Congenital Stationary, Type 1b