SDS-PAGE
S100A3 / S100E recombinant protein
Recombinant Human S100A3 / S100E Protein (His & MBP tag)
Gene Names
S100A3; S100E
Purity
> 95 % as determined by SDS-PAGE
Synonyms
S100A3 / S100E; Recombinant Human S100A3 / S100E Protein (His & MBP tag); S100E; S100A3 / S100E recombinant protein
Host
E Coli
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, 20% glycerol, pH 7.4
Sequence Length
101
Application Notes
The recombinant human S100A3/MBP fusion protein consists of 498 amino acids and has a calculated molecular mass of 55.3 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
Met
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for S100A3 / S100E recombinant protein
Background: Protein S100-A3, also known as Protein S-100E, S100 calcium-binding protein A3, S100A3 and S100E, is a member of the S-100 family. S100A3 / S100E contains 2 EF-hand domains. S100A3 / S100E is highly expressed in the differentiating cuticular cells within the hair follicle and organized into mature hair cuticles. High concentrations of S100A3 homotetramer might provide the millimolar level of Ca2+ required for hair cuticular barrier formation. S100A3 / S100E is a unique member of the Ca2+-binding S100 protein family with the highest cysteine content and affinity for Zn2+. S100A3 / S100E binds both calcium and zinc. S100A3 / S100E probably binds 2 zinc ions per molecule. It may be involved in calcium-dependent cuticle cell differentiation and hair shaft formation. S100A3 plays an important role in calcium-dependent processes leading to hair shaft formation. S100A3 / S100E is a unique protein among all members of the calcium-binding S100 family, is specifically expressed at the inner endocuticle of human hair fibers. Upon hair damage, S100A3 / S100E is released from hair fibers and possibly destabilizes the hair tissue architecture.
Description: A DNA sequence encoding the human S100A3 (P33764) (Met 1-Gln 101) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.
Description: A DNA sequence encoding the human S100A3 (P33764) (Met 1-Gln 101) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,713 Da
NCBI Official Full Name
protein S100-A3
NCBI Official Synonym Full Names
S100 calcium binding protein A3
NCBI Official Symbol
S100A3
NCBI Official Synonym Symbols
S100E
NCBI Protein Information
protein S100-A3
UniProt Protein Name
Protein S100-A3
UniProt Gene Name
S100A3
UniProt Synonym Gene Names
S100E
NCBI Description
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has the highest content of cysteines of all S100 proteins, has a high affinity for Zinc, and is highly expressed in human hair cuticle. The precise function of this protein is unknown. [provided by RefSeq, Jul 2008]
Uniprot Description
S100A3: Binds both calcium and zinc. Probably binds 2 zinc ions per molecule. May be involved in calcium-dependent cuticle cell differentiation and hair shaft formation. Belongs to the S-100 family.
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: cytoplasm; nucleolus
Research Articles on S100A3 / S100E
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Product Notes
The S100A3 / S100E s100a3 (Catalog #AAA2545907) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The recombinant human S100A3/MBP fusion protein consists of 498 amino acids and has a calculated molecular mass of 55.3 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the S100A3 / S100E s100a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A3 / S100E, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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