Human S100 calcium binding protein A2 ELISA Kit | S100A2 elisa kit

Human Protein S100-A2, S100A2 ELISA Kit

Cat. Num. AAA938477

• Gene Names: S100A2; CAN19; S100L
• Reactivity: Human
• Synonyms: S100 calcium binding protein A2; Human Protein S100-A2; S100A2 ELISA Kit; Human Protein S100-A2 (S100A2) ELISA kit; RP11-49N14.8; CAN19; MGC111539; S100L; OTTHUMP00000032968; OTTHUMP00000032969; S100 calcium-binding protein A2; S100A2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human S100A2. No significant cross-reactivity or interference between human S100A2 and analogues was observed.
• Sequence Length: 98

• Samples: Serum, plasma, tissue homogenates, cell lysates.
• Assay Type: Sandwich
• Detection Range: 1.56 ng/ml -100 ng/ml.
• Sensitivity: The minimum detectable dose of human S100A2 is typically less than 0.39 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5174661
• NCBI GeneID: 6273
• NCBI Accession #: NP_005969.1
• NCBI GenBank Nucleotide #: NM_005978.3
• UniProt Accession #: P29034; O00266; Q3KRB9; Q5RHS8; Q9BU83
• Molecular Weight: 11,117 Da
• NCBI Official Full Name: protein S100-A2
• NCBI Official Synonym Full Names: S100 calcium binding protein A2
• NCBI Official Symbol: S100A2
• NCBI Official Synonym Symbols: CAN19; S100L
• NCBI Protein Information: protein S100-A2; S100 calcium-binding protein A2
• UniProt Protein Name: Protein S100-A2
• Protein Family: Protein
• UniProt Gene Name: S100A2
• UniProt Synonym Gene Names: S100L
• UniProt Entry Name: S10A2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A2: May act as a modulator against excess calcium accumulation in normal human mammary epithelial cells. May also play a role in suppressing tumor cell growth. Belongs to the S-100 family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Molecular Function: identical protein binding; protein binding; calcium ion binding

Biological Process: endothelial cell migration