Western Blot (WB)
(Figure 1. Western blot analysis using ApoM mouse mAb against GST-ApoM recombinant protein (1) and human serum (2).)
Mouse anti-Human ApoM Antibody | anti-ApoM antibody
ApoM Antibody
Gene Names
APOM; G3a; NG20; apo-M; HSPC336
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
ApoM; ApoM Antibody; G3a; NG20; HSPC336; MGC22400; anti-ApoM antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Clone Number
10C3G5
Form/Format
Purified antibody in PBS containing 0.03% sodium azide.
Sequence Length
116
Applicable Applications for anti-ApoM antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000
IHC: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of ApoM expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Western Blot (WB)
(Figure 1. Western blot analysis using ApoM mouse mAb against GST-ApoM recombinant protein (1) and human serum (2).)
Western Blot (WB)
(Figure 1. Western blot analysis using ApoM mouse mAb against GST-ApoM recombinant protein (1) and human serum (2).)
Immunofluorescence (IF)
(Figure 2. Immunofluorescence analysis of methanol-fixed L-02 (left) and Cos7 (right) cells using ApoM mouse mAb showing cytoplasmic and membrane localization.)
Immunofluorescence (IF)
(Figure 2. Immunofluorescence analysis of methanol-fixed L-02 (left) and Cos7 (right) cells using ApoM mouse mAb showing cytoplasmic and membrane localization.)
Related Product Information for anti-ApoM antibody
ApoM (apolipoprotein M, also designated G3a or NG20), with 188-amino acid protein(about 21kDa), is an apolipoprotein and member of the lipocalin protein family. The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro, Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment. Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs, and also found in triglyceride-rich lipoproteins and LDLs.
Product Categories/Family for anti-ApoM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21kDa
NCBI Official Full Name
apolipoprotein M isoform 2
NCBI Official Synonym Full Names
apolipoprotein M
NCBI Official Symbol
APOM
NCBI Official Synonym Symbols
G3a; NG20; apo-M; HSPC336
NCBI Protein Information
apolipoprotein M
UniProt Protein Name
Apolipoprotein M
Protein Family
UniProt Gene Name
APOM
UniProt Synonym Gene Names
G3A; NG20; Apo-M; ApoM
NCBI Description
The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
Research Articles on ApoM
Similar Products
Product Notes
The ApoM apom (Catalog #AAA850188) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ApoM Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ApoM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the ApoM apom for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ApoM, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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