Human Protein S100-A10 ELISA Kit | S100A10 elisa kit

Human Protein S100-A10 ELISA Kit

Cat. Num. AAA765759

• Gene Names: S100A10; 42C; P11; p10; GP11; ANX2L; CAL1L; CLP11; Ca[1]; ANX2LG
• Reactivity: Human
• Synonyms: Protein S100-A10; Human Protein S100-A10 ELISA Kit; S100A10/annexin II ligand; calpactin I; light polypeptide/ANX2L/ANX2LG/CAL1LGP11; 42C/Calpactin I light chain/Calpactin-1 light chain/Cellular ligand of annexin II/CLP11Ca[1]/MGC111133/p10/p10 protein/p11/P11/protein S100-A10/S100 calcium binding protein A10/S100 calcium binding protein A10 (annexin II ligand; lightpolypeptide (p11))/S100 calcium-binding protein A10/S100 calcium-binding protein A10 (annexin II ligand; lightpolypeptide (p11)); S100A10 elisa kit

• Reactivity: Human
• Sequence Length: 97

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for S100A10 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology anti-Human S100A10 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human S100A10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human S100A10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human S100A10 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506761
• NCBI GeneID: 6281
• NCBI Accession #: NP_002957.1
• NCBI GenBank Nucleotide #: NM_002966.2
• Molecular Weight: 11,203 Da
• NCBI Official Full Name: protein S100-A10
• NCBI Official Synonym Full Names: S100 calcium binding protein A10
• NCBI Official Symbol: S100A10
• NCBI Official Synonym Symbols: 42C; P11; p10; GP11; ANX2L; CAL1L; CLP11; Ca[1]; ANX2LG
• NCBI Protein Information: protein S100-A10
• UniProt Protein Name: Protein S100-A10
• Protein Family: Protein
• UniProt Gene Name: S100A10
• UniProt Synonym Gene Names: ANX2LG; CAL1L; CLP11
• UniProt Entry Name: S10AA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A10: Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine- specific kinase. Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with SCN10A. Belongs to the S-100 family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extrinsic to plasma membrane; lipid raft

Molecular Function: calcium ion binding; lipid binding; protein binding; protein homodimerization activity

Biological Process: lipid raft formation; membrane budding; positive regulation of binding; positive regulation of focal adhesion formation; positive regulation of GTPase activity; positive regulation of stress fiber formation; protein heterotetramerization