SDS-Page
(RXR-LBD protein gel. RXR-LBD run on an SDS-PAGE gel and stained with Coomassie blue.)
RXR-LBD recombinant protein
Recombinant RXR-LBD protein
Synonyms
RXR-LBD; Recombinant RXR-LBD protein; RAR; NR1B1; RXR-LBD recombinant protein
Host
E Coli
Form/Format
100ug of recombinant RXR-LBD protein expressed in E Coli at a concentration of 5.49mg/ml in 50mM Tris pH8.0, 150mM NaCl, 1mM DTT and 50% glycerol.
Protein Species
Human
Notes
Recombinant RXR-LBD is suitable for in vitro transcription, ligand binding and protein-protein interaction assays. 20ng is sufficient for in vitro transcription assays and 100ng is sufficient for ligand binding and protein-protein interaction studies.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(RXR-LBD protein gel. RXR-LBD run on an SDS-PAGE gel and stained with Coomassie blue.)
SDS-Page
(RXR-LBD protein gel. RXR-LBD run on an SDS-PAGE gel and stained with Coomassie blue.)
Related Product Information for RXR-LBD recombinant protein
Short Description: RXR-a is a nuclear receptor that forms heterodimers with retinoic acid receptors (RAR). The ligand binding domain (LBD) corresponding to amino acids 223-463 of RXR-a (accession number 1609194A) was expressed in E Coli with an apparent molecular weight of 27 kDa. An epitope tag present during expression was removed proteolytically after purification. The Retinoid X receptor (RXR) ligand binding domain (LBD) is a promiscuous heterodimerization domain for binding of RXR to many nuclear receptors, including thyroid hormone receptors (TRs), retinoic acid receptors (RARs), peroxisome proliferator-activated receptor, orphan nuclear receptors and oxysterol receptors. Binding of RXR-LBD to nuclear receptors is mediated by the identity box, a 40-amino acid subregion within the ligand binding domain. The LBD is responsible for mediating transcription through receptor homo- and heterodimerization. The specific function of the RXR-containing complexes in regulating transcription depends on the associated ligand. For example, hormone binding to the LBD of the RXR results a conformational change that affects the C-terminal transactivation helix H12 involved in transcriptional activation. RXR-LBD ligand binding triggers dissociation of corepressor and the recruitment of coactivators, such as members of the Src family kinases, NCOA2/GRIP1, and RAC3/AIB1 which, in turn, promote transcription of downstream targets. Recombinant RXR-LBD is for in vitro transcription, ligand binding and protein interaction assays.
Background: The Retinoid X receptor (RXR) ligand binding domain (LBD) is a promiscuous heterodimerization domain for binding of RXR to many nuclear receptors, including thyroid hormone receptors (TRs), retinoic acid receptors (RARs), peroxisome proliferator-activated receptor, orphan nuclear receptors and oxysterol receptors. Binding of RXR-LBD to nuclear receptors is mediated by the identity box, a 40-amino acid subregion within the ligand binding domain. The LBD is responsible for mediating transcription through receptor homo- and heterodimerization. The specific function of the RXR-containing complexes in regulating transcription depends on the associated ligand. For example, hormone binding to the LBD of the RXR results a conformational change that affects the C-terminal transactivation helix H12 involved in transcriptional activation. RXR-LBD ligand binding triggers dissociation of corepressor and the recruitment of coactivators, such as members of the Src family kinases, NCOA2/GRIP1, and RAC3/AIB1 which, in turn, promote transcription of downstream targets.
Background: The Retinoid X receptor (RXR) ligand binding domain (LBD) is a promiscuous heterodimerization domain for binding of RXR to many nuclear receptors, including thyroid hormone receptors (TRs), retinoic acid receptors (RARs), peroxisome proliferator-activated receptor, orphan nuclear receptors and oxysterol receptors. Binding of RXR-LBD to nuclear receptors is mediated by the identity box, a 40-amino acid subregion within the ligand binding domain. The LBD is responsible for mediating transcription through receptor homo- and heterodimerization. The specific function of the RXR-containing complexes in regulating transcription depends on the associated ligand. For example, hormone binding to the LBD of the RXR results a conformational change that affects the C-terminal transactivation helix H12 involved in transcriptional activation. RXR-LBD ligand binding triggers dissociation of corepressor and the recruitment of coactivators, such as members of the Src family kinases, NCOA2/GRIP1, and RAC3/AIB1 which, in turn, promote transcription of downstream targets.
Product Categories/Family for RXR-LBD recombinant protein
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Molecular weight of 27kDa
Similar Products
Product Notes
The RXR-LBD (Catalog #AAA388086) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RXR-LBD, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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