RNA-splicing ligase RtcB Recombinant Protein | rtcB recombinant protein

Recombinant Escherichia coli RNA-splicing ligase RtcB

Cat. Num. AAA1175065

• Gene Names: rtcB; ECK3407; JW3384; yhgL
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: RNA-splicing ligase RtcB; Recombinant Escherichia coli RNA-splicing ligase RtcB; rtcB recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-408aa; Full Length
• Sequence: MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIAVMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQAIETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHLGTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRDLAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVEVIYTLRQVVCVKG
• Sequence Length: 408

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for rtcB recombinant protein

RNA ligase that mediates the joining of broken tRNA-like st-loop structures in case of tRNA damage. Probably participates to tRNA restriction-repair by ligating broken tRNA-like st-loop structures with 2',3'-cyclic phosphate and 5'-OH ends to form a splice junction with a 2'-OH, 3',5'-phosphodiester, a step that requires GTP. Also acts as a DNA ligase in case of DNA damage by splicing 'dirty' DNA breaks, characterized by 3'-PO4 (or cyclic-PO4) and 5'-OH ends that cannot be sealed by classical DNA ligases.

Product Categories/Family for rtcB recombinant protein

Cell Biology

References

The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon.Genschik P., Drabikowski K., Filipowicz W.J. Biol. Chem. 273:25516-25526(1998) RtcB is the RNA ligase component of an Escherichia coli RNA repair operon.Tanaka N., Shuman S.J. Biol. Chem. 286:7727-7731(2011) RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo.Tanaka N., Meineke B., Shuman S.J. Biol. Chem. 286:30253-30257(2011) Novel mechanism of RNA repair by RtcB via sequential 2',3'-cyclic phosphodiesterase and 3'-Phosphate/5'-hydroxyl ligation reactions.Tanaka N., Chakravarty A.K., Maughan B., Shuman S.J. Biol. Chem. 286:43134-43143(2011) The sequential 2',3'-cyclic phosphodiesterase and 3'-phosphate/5'-OH ligation steps of the RtcB RNA splicing pathway are GTP-dependent.Chakravarty A.K., Shuman S.Nucleic Acids Res. 40:8558-8567(2012) RNA ligase RtcB splices 3'-phosphate and 5'-OH ends via covalent RtcB-(histidinyl) -GMP and polynucleotide-(3') pp(5') G intermediates.Chakravarty A.K., Subbotin R., Chait B.T., Shuman S.Proc. Natl. Acad. Sci. U.S.A. 109:6072-6077(2012) Rewriting the rules for end joining via enzymatic splicing of DNA 3'-PO4 and 5'-OH ends.Das U., Chakravarty A.K., Remus B.S., Shuman S.Proc. Natl. Acad. Sci. U.S.A. 110:20437-20442(2013)

NCBI and Uniprot Product Information

• NCBI GI #: 16131295
• NCBI GeneID: 947929
• NCBI Accession #: NP_417879.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P46850; P76690; Q2M785
• Molecular Weight: 47.2 kDa
• NCBI Official Full Name: RNA-splicing ligase
• NCBI Official Symbol: rtcB
• NCBI Official Synonym Symbols: ECK3407; JW3384; yhgL
• NCBI Protein Information: RNA-splicing ligase
• UniProt Protein Name: RNA-splicing ligase RtcB
• Protein Family: RNA-splicing ligase
• UniProt Gene Name: rtcB
• UniProt Synonym Gene Names: yhgL
• UniProt Entry Name: RTCB_ECOLI

NCBI Description

RtcB has similarity to proteins found in Eucarya, Bacteria, and Archaea . [More information is available at EcoCyc: G7751].

Uniprot Description

RNA ligase that mediates the joining of broken tRNA-like stem-loop structures in case of tRNA damage. Probably participates to tRNA restriction-repair by ligating broken tRNA-like stem-loop structures with 2',3'-cyclic phosphate and 5'-OH ends to form a splice junction with a 2'-OH, 3',5'-phosphodiester, a step that requires GTP (PubMed:21224389, PubMed:22730297). Also acts as a DNA ligase in case of DNA damage by splicing 'dirty' DNA breaks, characterized by 3'-PO4 (or cyclic-PO4) and 5'-OH ends that cannot be sealed by classical DNA ligases (PubMed:24218597).