Putative ribosome biogenesis GTPase RsgA (rsgA) Recombinant Protein | rsgA recombinant protein

Recombinant Escherichia coli Putative ribosome biogenesis GTPase RsgA (rsgA)

Cat. Num. AAA1089377

• Gene Names: rsgA; ECK4157; engC; JW4122; yjeQ
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Putative ribosome biogenesis GTPase RsgA (rsgA); Recombinant Escherichia coli Putative ribosome biogenesis GTPase RsgA (rsgA); rsgA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-350, full length protein
• Sequence: MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIVISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHERTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLNKIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGVGKSSLLNALLGLQKEILTNDISDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLWHLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILESMAQVKTRKNFSDTDD
• Sequence Length: 350

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 90111696
• NCBI GeneID: 948674
• NCBI Accession #: NP_418585.4
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P39286; Q2M6E1
• Molecular Weight: 39,193 Da
• NCBI Official Full Name: ribosome small subunit-dependent GTPase A
• NCBI Official Symbol: rsgA
• NCBI Official Synonym Symbols: ECK4157; engC; JW4122; yjeQ
• NCBI Protein Information: ribosome small subunit-dependent GTPase A
• UniProt Protein Name: Small ribosomal subunit biogenesis GTPase RsgA
• Protein Family: Putative ribosome biogenesis GTPase
• UniProt Gene Name: rsgA

NCBI Description

rsgA(yjeQ) has been reported to be an essential gene, but deletion mutants have also been reported to grow slowly. [More information is available at EcoGene: EG12479]. Based on genetic interactions, RgsA/YjeQ is thought to play a role in late ribosome biogenesis . [More information is available at EcoCyc: G7841].

Uniprot Description

One of at least 4 proteins (Era, RbfA, RimM and RsgA/YjeQ) that assist in the late maturation steps of the functional core of the 30S ribosomal subunit (PubMed:18223068, PubMed:21102555, PubMed:21303937, PubMed:25904134, PubMed:27382067). Binds the 30S subunit contacting the head, platform, and rRNA helix 44, which may assist the last maturation stages (PubMed:21788480, PubMed:21960487). Removes RbfA from mature, but not immature 30S ribosomes in a GTP-dependent manner; 95% removal in the presence of GTP, 90% removal in GMP-PNP and 65% removal in the presence of GDP (PubMed:21102555, PubMed:25904134). Circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover (PubMed:12220175). Dispensible for viability, but important for overall fitness. The intrinsic GTPase activity is stimulated by the presence of 30S (160-fold increase in kcat) or 70S (96-fold increase in kcat) ribosomes (PubMed:14973029). Mature 30S ribosomes stimulate intrinsic GTPase more than do immature 30S ribosomes (PubMed:25904134). Ribosome-associated GTPase activity is stimulated by RbfA (PubMed:21102555). The GTPase is inhibited by aminoglycoside antibiotics such as neomycin and paromycin (PubMed:15466596) streptomycin and spectinomycin (PubMed:15828870). This inhibition is not due to competition for binding sites on the 30S or 70S ribosome (PubMed:15828870).