Ribosome Binding Protein 1 (RRBP1) Recombinant Protein | RRBP1 recombinant protein

Recombinant Ribosome Binding Protein 1 (RRBP1)

Cat. Num. AAA2086817

• Gene Names: RRBP1; RRp; hES; ES130; ES/130
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Ribosome Binding Protein 1 (RRBP1); Recombinant Ribosome Binding Protein 1 (RRBP1); ES/130; ES130; hES; RRp; 180 kDa ribosome receptor homolog; ES/130-related protein; Ribosome receptor protein; RRBP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 977

• Applicable Applications for RRBP1 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Arg822~Val977
• Tag: N-terminal His-tag
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Prokaryotic expression
• Organism Species: Human
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 4.7
• Subcellular Location: Endoplasmic reticulum membrane; Single-pass type III membrane protei
• Usage: Reconstitute in PBS or others.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Product Categories/Family for RRBP1 recombinant protein

Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 110611218
• NCBI GeneID: 6238
• NCBI Accession #: NP_001036041.1
• NCBI GenBank Nucleotide #: NM_001042576.1
• UniProt Accession #: Q9P2E9; O75300; O75301; Q5W165; Q96SB2; Q9BWP1; Q9H476; A2A2S6; A6NCN6
• Molecular Weight: 21.1kDa
• NCBI Official Full Name: ribosome-binding protein 1
• NCBI Official Synonym Full Names: ribosome binding protein 1
• NCBI Official Symbol: RRBP1
• NCBI Official Synonym Symbols: RRp; hES; ES130; ES/130
• NCBI Protein Information: ribosome-binding protein 1
• UniProt Protein Name: Ribosome-binding protein 1
• Protein Family: Ribosome-binding protein
• UniProt Gene Name: RRBP1
• UniProt Synonym Gene Names: KIAA1398; RRp

NCBI Description

This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012]

Uniprot Description

RRBP1: a single-pass type III membrane protein of the rough endoplasmic reticulum membrane. Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. Associated with the unfolded protein response (UPR) and has anti-apoptotic activity. Knockdown of RRBP1 induces ER stress, and is associated with a significant reduction in the expression of glucose-regulated protein 78 (GRP78). GRP78 regulates the UPR, is widely upregulated in cancer, and plays an important role in chemotherapy resistance in some cancers. Upregulated in many cancers: overexpressed in a high proportion of breast carcinoma cases tested, and is highly expressed in lung cancer tissue compared with adjacent normal tissues. Because RRBP1 appears to play a role in tumor cell survival, it may be a candidate for new targeted therapeutics. Three human isoforms are produced by alternative splicing.

Protein type: Membrane protein, integral; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 20p12.1

Cellular Component: endoplasmic reticulum; integral to endoplasmic reticulum membrane; membrane; ribosome

Molecular Function: receptor activity

Biological Process: osteoblast differentiation; translation