Rabbit anti-Human, Mouse GTF2B Polyclonal Antibody | anti-GTF2B antibody

GTF2B, NT (GTF2B, TF2B, TFIIB, Transcription initiation factor IIB, General transcription factor TFIIB, S300-II) (FITC)

Cat. Num. AAA6304825

• Gene Names: GTF2B; TF2B; TFIIB
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GTF2B; Polyclonal Antibody; NT (GTF2B; TF2B; TFIIB; Transcription initiation factor IIB; General transcription factor TFIIB; S300-II) (FITC); S300-II; anti-GTF2B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GTF2B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GTF2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the N-terminal region of human GTF2B.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GTF2B (arrow) using rabbit polyclonal GTF2B Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2B gene.)

Western Blot (WB)

(GTF2B Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the GTF2B antibody detected the GTF2B protein (arrow).)

Related Product Information for anti-GTF2B antibody

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.

Product Categories/Family for anti-GTF2B antibody

Antibodies; Transcription Factors

References

Wang, Y., et al. Curr. Biol. 20(6):548-553(2010). Thompson, N.E., et al. J. Biol. Chem. 284(37):24754-24766(2009). Gilman, B., et al. J. Biol. Chem. 284(14):9093-9098(2009). Vogt, C., et al. J. Virol. 82(22):11446-11453(2008). Elsby, L.M., et al. EMBO Rep. 7(9):898-903(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 169259765
• NCBI GeneID: 2959
• NCBI Accession #: NP_001505.1
• NCBI GenBank Nucleotide #: NM_001514
• UniProt Accession #: Q00403; Q5JS30; A8K1A7
• Molecular Weight: 34,833 Da
• NCBI Official Full Name: Homo sapiens general transcription factor IIB (GTF2B), mRNA
• NCBI Official Synonym Full Names: general transcription factor IIB
• NCBI Official Symbol: GTF2B
• NCBI Official Synonym Symbols: TF2B; TFIIB
• NCBI Protein Information: transcription initiation factor IIB; RNA polymerase II transcription factor IIB; S300-II; general transcription factor TFIIB
• UniProt Protein Name: Transcription initiation factor IIB
• Protein Family: Transcription initiation factor
• UniProt Gene Name: GTF2B
• UniProt Synonym Gene Names: TF2B; TFIIB
• UniProt Entry Name: TF2B_HUMAN

NCBI Description

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]

Uniprot Description

GTF2B: General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. Associates with TFIID-IIA (DA complex) to form TFIID-IIA- IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1. Interacts with Epstein-Barr virus EBNA2. Belongs to the TFIIB family.

Protein type: Transcription factor; Nuclear receptor co-regulator; Acetyltransferase

Chromosomal Location of Human Ortholog: 1p22-p21

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding; zinc ion binding; TATA-binding protein binding; thyroid hormone receptor binding; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase II promoter; gene expression