Ubiquitin-40S ribosomal protein S27a (RPS27A) Recombinant Protein | RPS27A recombinant protein

Recombinant Guinea pig Ubiquitin-40S ribosomal protein S27a (RPS27A)

Cat. Num. AAA956930

• Gene Names: Rps27a; Ubiz
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ubiquitin-40S ribosomal protein S27a (RPS27A); Recombinant Guinea pig Ubiquitin-40S ribosomal protein S27a (RPS27A); RPS27A recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-76, Full length protein
• Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
• Sequence Length: 76

• Species: Cavia porcellus (Guinea pig)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for RPS27A recombinant protein

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 290543388
• NCBI GeneID: 100286787
• NCBI Accession #: NP_001166537.1
• NCBI GenBank Nucleotide #: NM_001173066.1
• UniProt Accession #: P62978; P02248; P02249; P02250; P14798; P62977; Q29120; Q91887; Q91888; Q9BQ77
• Molecular Weight: 17,965 Da
• NCBI Official Full Name: ubiquitin-40S ribosomal protein S27a
• NCBI Official Symbol: Rps27a
• NCBI Official Synonym Symbols: Ubiz
• NCBI Protein Information: ubiquitin-40S ribosomal protein S27a
• UniProt Protein Name: Ubiquitin-40S ribosomal protein S27a
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS27A
• UniProt Synonym Gene Names: UBA80; UBCEP1; UBIZ

Uniprot Description

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling ().