Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD34 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate)
Mouse anti-Human CD34 Monoclonal Antibody | anti-CD34 antibody
CD34 antibody
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
By protein-A affinity chromatography
Synonyms
CD34; Monoclonal Antibody; CD34 antibody; Hematopoietic progenitor cell antigen CD34 isoform b; anti-CD34 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT3B6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
328
Applicable Applications for anti-CD34 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Antigen Species
Human
Immunogen
Recombinant human CD34 (32-290aa) purified from Baculovirus.
Clone Note
Anti-human CD34 mAb, clone AT3B6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD34 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD34 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate)
Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD34 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate)
Related Product Information for anti-CD34 antibody
CD34, also known as hematopoietic progenitor cell antigen CD34 isoform b, is a member of a family of single-pass transmembrane sialomucin proteins. It is adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. This protein could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.
Product Categories/Family for anti-CD34 antibody
References
Nielsen JS., et al. (2009) Microcirculation. 16: 487-496.; Mastrandrea F., et al. (2008) Eur Ann Allergy Clin Immunol. 40: 90-103.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,011 Da
NCBI Official Full Name
hematopoietic progenitor cell antigen CD34 isoform b
NCBI Official Synonym Full Names
CD34 molecule
NCBI Official Symbol
CD34
NCBI Protein Information
hematopoietic progenitor cell antigen CD34
UniProt Protein Name
Hematopoietic progenitor cell antigen CD34
Protein Family
UniProt Gene Name
CD34
NCBI Description
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Research Articles on CD34
Similar Products
Product Notes
The CD34 cd34 (Catalog #AAA206613) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD34 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD34 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD34 cd34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD34, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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