SDS-Page
RPP30 recombinant protein
RPP30, 1-268aa, Human, His tag, E Coli
Gene Names
RPP30; TSG15
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
RPP30; 1-268aa; Human; His tag; E Coli; Ribonuclease P protein subunit p30; RNASEP2; TSG15; RPP30 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200mM NaCl, 1mM EDTA
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAVFADL DLRAGSDLKA LRGLVETAAH LGYSVVAINH IVDFKEKKQE IEKPVAVSEL FTTLPIVQGK SRPIKILTRL TIIVSDPSHC NVLRATSSRA RLYDVVAVFP KTEKLFHIAC THLDVDLVCI TVTEKLPFYF KRPPINVAID RGLAFELVYS PAIKDSTMRR YTISSALNLM QICKGKNVII SSAAERPLEI RGPYDVANLG LLFGLSESDA KAAVSTNCRA ALLHGETRKT AFGIISTVKK PRPSEGDEDC LPASKKAKCE G
Sequence Length
268
Applicable Applications for RPP30 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for RPP30 recombinant protein
RPP30 (Ribonuclease P protein subunit p30) belongs to the eukaryotic/archaeal RNase P protein component 3 family. RPP30 is component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ribonuclease P (PNase P) is small nuclear ribonucleoproteins (snRNPs) that act on RNA substrates in vitro. Also, RNase P accumulates in the nucleolus, have a similar RNA component and also have several protein subunits in common. Recombinant human RPP30 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for RPP30 recombinant protein
References
Kurz, J.C., et al. (2000) Curr. Opin. Chem. Biol. 4: 553-558.; van Eenennaam, H., et al. (2001) Mol. Biol. Cell. 12: 3680-3689.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31.8kDa (291aa), confirmed by MALDI-TOF
NCBI Official Full Name
ribonuclease P protein subunit p30 isoform b
NCBI Official Synonym Full Names
ribonuclease P/MRP subunit p30
NCBI Official Symbol
RPP30
NCBI Official Synonym Symbols
TSG15
NCBI Protein Information
ribonuclease P protein subunit p30
UniProt Protein Name
Ribonuclease P protein subunit p30
Protein Family
UniProt Gene Name
RPP30
UniProt Synonym Gene Names
RNASEP2; RNaseP protein p30
Uniprot Description
Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.
Research Articles on RPP30
Similar Products
Product Notes
The RPP30 rpp30 (Catalog #AAA204021) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's RPP30 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the RPP30 rpp30 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMAVFADL DLRAGSDLKA LRGLVETAAH LGYSVVAINH IVDFKEKKQE IEKPVAVSEL FTTLPIVQGK SRPIKILTRL TIIVSDPSHC NVLRATSSRA RLYDVVAVFP KTEKLFHIAC THLDVDLVCI TVTEKLPFYF KRPPINVAID RGLAFELVYS PAIKDSTMRR YTISSALNLM QICKGKNVII SSAAERPLEI RGPYDVANLG LLFGLSESDA KAAVSTNCRA ALLHGETRKT AFGIISTVKK PRPSEGDEDC LPASKKAKCE G. It is sometimes possible for the material contained within the vial of "RPP30, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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