SDS-Page
(3ug by SDS-PAGE under reducing condition and visualization by coomassie blue stain.)
EIF1AX recombinant protein
EIF1AX, 1-144aa, Human, His tag, E Coli
Gene Names
EIF1AX; EIF1A; EIF4C; eIF-1A; eIF-4C; EIF1AP1
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
EIF1AX; 1-144aa; Human; His tag; E Coli; Eukaryotic translation initiation factor 1A X-linked; eIF-1A; eIF-4C; EIF1A; EIF1AP1; EIF4C; EIF1AX recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 40% glycerol, 5mM DTT, 200mM NaCl
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MPKNKGKGGK NRRRGKNENE SEKRELVFKE DGQEYAQVIK MLGNGRLEAM CFDGVKRLCH IRGKLRKKVW INTSDIILVG LRDYQDNKAD VILKYNADEA RSLKAYGELP EHAKINETDT FGPGDDDEIQ FDDIGDDDED IDDI
Sequence Length
144
Applicable Applications for EIF1AX recombinant protein
SDS-PAGE
Tag Information
His-tag
Preparation and Storage
Can be stored at +2°C to +8°C for one week. For long term storage , aliquot and store at -20°C to -80°C
Avoid repeated freezing and thawing cycles.
Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualization by coomassie blue stain.)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualization by coomassie blue stain.)
Related Product Information for EIF1AX recombinant protein
EIF1AX is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. It seems to be required for maximal rate of protein biosynthesis. And it enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA (I) to 40 S ribosomal subunits. Recombinant human EIF1AX protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for EIF1AX recombinant protein
References
Lahn BT., et al. (1997) Science 278 (5338):675-80.; Kim S., et al. (2007) Stem Cells Dev. 16 (4):537-45.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18.6kDa (164aa), confirmed by MALDI-TOF
NCBI Official Full Name
eukaryotic translation initiation factor 1A, X-chromosomal
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 1A, X-linked
NCBI Official Symbol
EIF1AX
NCBI Official Synonym Symbols
EIF1A; EIF4C; eIF-1A; eIF-4C; EIF1AP1
NCBI Protein Information
eukaryotic translation initiation factor 1A, X-chromosomal
UniProt Protein Name
Eukaryotic translation initiation factor 1A, X-chromosomal
Protein Family
UniProt Gene Name
EIF1AX
UniProt Synonym Gene Names
EIF1A; EIF4C; eIF-1A X isoform; eIF-4C
NCBI Description
This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq, Jul 2008]
Uniprot Description
Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.
Research Articles on EIF1AX
Similar Products
Product Notes
The EIF1AX eif1ax (Catalog #AAA203707) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's EIF1AX can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the EIF1AX eif1ax for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MPKNKGKGGK NRRRGKNENE SEKRELVFKE DGQEYAQVIK MLGNGRLEAM CFDGVKRLCH IRGKLRKKVW INTSDIILVG LRDYQDNKAD VILKYNADEA RSLKAYGELP EHAKINETDT FGPGDDDEIQ FDDIGDDDED IDDI. It is sometimes possible for the material contained within the vial of "EIF1AX, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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