Immunohistochemistry (IHC)
(Biotinylated (0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
ribosomal protein L8 Antibody | anti-RPL8 antibody
Goat anti-ribosomal protein L8, Biotinylated Antibody
Expected from sequence similarity: Mouse, Rat, Dog
Expected from sequence similarity: Mouse, Rat, Dog
WB: Approx 28kDa band observed in lysates of cell line HepG2. Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry (IHC)
(Biotinylated (0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Immunohistochemistry (IHC)
(Biotinylated (0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Uniprot Description
Component of the large ribosomal subunit.
Research Articles on RPL8
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Product Notes
The RPL8 rpl8 (Catalog #AAA423721) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ribosomal protein L8, Biotinylated Antibody reacts with Human Expected from sequence similarity: Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's ribosomal protein L8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Pep-ELISA: antibody detection limit dilution 1:128000. WB: Approx 28kDa band observed in lysates of cell line HepG2. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the RPL8 rpl8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ribosomal protein L8, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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