Retinoid isomerohydrolase (RPE65) Recombinant Protein | RPE65 recombinant protein

Recombinant Bovine Retinoid isomerohydrolase (RPE65) , partial

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Retinoid isomerohydrolase (RPE65); Recombinant Bovine Retinoid isomerohydrolase (RPE65); partial; RPE65 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 533

• Species: Bovine
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for RPE65 recombinant protein

This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.

NCBI and Uniprot Product Information

• NCBI GI #: 27806125
• NCBI GeneID: 282043
• NCBI Accession #: NP_776878.1
• NCBI GenBank Nucleotide #: NM_174453.2
• UniProt Accession #: Q28175; Q05661
• Molecular Weight: 60,944 Da
• NCBI Official Full Name: retinoid isomerohydrolase
• NCBI Official Synonym Full Names: RPE65, retinoid isomerohydrolase
• NCBI Official Symbol: RPE65
• NCBI Protein Information: retinoid isomerohydrolase
• UniProt Protein Name: Retinoid isomerohydrolase
• UniProt Gene Name: RPE65

Uniprot Description

Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:16096063, PubMed:19805034, PubMed:20100834). Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid (). The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (PubMed:15186777).