Rat Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit | ALAS1 elisa kit

Rat Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit

Cat. Num. AAA2705715

• Gene Names: ALAS1; ALAS; MIG4; ALAS3; ALASH; ALAS-H
• Reactivity: Rat
• Synonyms: Aminolevulinate Delta Synthase 1 (ALAS1); Rat Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit; ALAS3; ALAS; ALASH; 5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; 5-aminolevulinate synthase; nonspecific; mitochondrial; ALAS1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aminolevulinate Delta Synthase 1 (ALAS1).; No significant cross-reactivity or interference between Aminolevulinate Delta Synthase 1 (ALAS1) and analogues was observed.
• Sequence Length: 118

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.36ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aminolevulinate Delta Synthase 1 (ALAS1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aminolevulinate Delta Synthase 1 (ALAS1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALAS1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminolevulinate Delta Synthase 1 (ALAS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminolevulinate Delta Synthase 1 (ALAS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminolevulinate Delta Synthase 1 (ALAS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aminolevulinate Delta Synthase 1 (ALAS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21104488
• NCBI GeneID: 211
• UniProt Accession #: P13196
• Molecular Weight: 12,961 Da
• NCBI Official Full Name: aminolevulinate delta-synthase 1
• NCBI Official Synonym Full Names: 5'-aminolevulinate synthase 1
• NCBI Official Symbol: ALAS1
• NCBI Official Synonym Symbols: ALAS; MIG4; ALAS3; ALASH; ALAS-H
• NCBI Protein Information: 5-aminolevulinate synthase, nonspecific, mitochondrial
• UniProt Protein Name: 5-aminolevulinate synthase, nonspecific, mitochondrial
• Protein Family: Alanine--tRNA ligase
• UniProt Gene Name: ALAS1
• UniProt Synonym Gene Names: ALAS3; ALASH; ALAS-H

NCBI Description

This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]

Uniprot Description

MiscellaneousThere are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.