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SDS-Page (SDS: Analysis of RPA3 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.)

RPA3 recombinant protein

RPA3 (Replication Protein A3, 14kD) Recombinant

Gene Names
RPA3; REPA3
Applications
ELISA
Purity
Highly Purified
Highly purified (95%)
Synonyms
RPA3; RPA3 (Replication Protein A3; 14kD) Recombinant; RPA3 recombinant protein
Ordering
For Research Use Only!
Purity/Purification
Highly Purified
Highly purified (95%)
Form/Format
Supplied as a liquid in 10mM Tris,, pH 8.0, 0.1% triton X-100, 0.002% sodium azide, 10mM DTT.
Sequence
121aa, Full-length RPA3
Applicable Applications for RPA3 recombinant protein
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA, Western Blot and MS.
Tag Fusion Partner
His-tag at N-terminus
Quality Control
SDS-PAGE
Preparation and Storage
For long-term storage, aliquot to avoid repeated freezing and thawing. Freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

SDS-Page

(SDS: Analysis of RPA3 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.)

SDS-Page (SDS: Analysis of RPA3 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.)
Related Product Information for RPA3 recombinant protein
The 14kD subunit of human RPA was cloned from HeLa cell cDNA library. The RPA3 gene has a 692bp sequence with an open reading frame encoding a protein of 121 amino acids. The deduced amino acid sequence demonstrates limited similiarity to the small subunit of yeast RPA from a HeLa cell cDNA library.
Product Categories/Family for RPA3 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,569 Da
NCBI Official Full Name
replication protein A 14 kDa subunit
NCBI Official Synonym Full Names
replication protein A3, 14kDa
NCBI Official Symbol
RPA3
NCBI Official Synonym Symbols
REPA3
NCBI Protein Information
replication protein A 14 kDa subunit; RP-A p14; RF-A protein 3; replication factor A protein 3
UniProt Protein Name
Replication protein A 14 kDa subunit
Protein Family
UniProt Gene Name
RPA3
UniProt Synonym Gene Names
REPA3; RPA14; RP-A p14
UniProt Entry Name
RFA3_HUMAN

Uniprot Description

RPA3: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: nucleoplasm; DNA replication factor A complex

Molecular Function: protein binding; damaged DNA binding; single-stranded DNA binding

Biological Process: mismatch repair; regulation of mitotic cell cycle; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; regulation of cell proliferation; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

Research Articles on RPA3

Similar Products

Product Notes

The RPA3 rpa3 (Catalog #AAA637851) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RPA3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA, Western Blot and MS. Researchers should empirically determine the suitability of the RPA3 rpa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 121aa, Full-lengt h RPA3. It is sometimes possible for the material contained within the vial of "RPA3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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