Replication protein A 32 kDa subunit Recombinant Protein | RFA2 recombinant protein

Recombinant Human Replication protein A 32 kDa subunit

Cat. Num. AAA9421578

• Gene Names: RPA2; REPA2; RPA32; RP-A p32; RP-A p34
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Replication protein A 32 kDa subunit; Recombinant Human Replication protein A 32 kDa subunit; Replication factor A protein 2; RF-A protein 2Replication protein A 34 kDa subunit; RP-A p34; RFA2 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Partial of the Full Length of 1-270aa, 1-267aa

• Tag Info: GST-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for RFA2 recombinant protein

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance

NCBI and Uniprot Product Information

• NCBI GI #: 554595586
• NCBI GeneID: 6118
• NCBI Accession #: NP_001273005.1
• NCBI GenBank Nucleotide #: NM_001286076.1
• UniProt Accession #: P15927; Q52II0; Q5TEI9; Q5TEJ5
• Molecular Weight: 56.3kD
• NCBI Official Full Name: replication protein A 32 kDa subunit isoform 2
• NCBI Official Synonym Full Names: replication protein A2
• NCBI Official Symbol: RPA2
• NCBI Official Synonym Symbols: REPA2; RPA32; RP-A p32; RP-A p34
• NCBI Protein Information: replication protein A 32 kDa subunit
• UniProt Protein Name: Replication protein A 32 kDa subunit
• Protein Family: Replication protein
• UniProt Gene Name: RPA2
• UniProt Synonym Gene Names: REPA2; RPA32; RPA34; RP-A p32; RF-A protein 2; RP-A p34

Uniprot Description

RFA2: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: chromatin; chromosome, telomeric region; DNA replication factor A complex; nuclear body; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body

Molecular Function: damaged DNA binding; enzyme binding; protein binding; protein phosphatase binding; single-stranded DNA binding; ubiquitin protein ligase binding

Biological Process: base-excision repair; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA replication; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1 DNA damage checkpoint; G1/S transition of mitotic cell cycle; mismatch repair; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair