Human Rho-associated, coiled-coil containing protein kinase 2 ELISA Kit | ROCK2 elisa kit

Human Rho-associated coiled-coil containing protein kinase 2, Rock-2 ELISA Kit

Cat. Num. AAA705283

• Gene Names: ROCK2; ROCK-II
• Reactivity: Human
• Synonyms: Rho-associated; coiled-coil containing protein kinase 2; Human Rho-associated coiled-coil containing protein kinase 2; Rock-2 ELISA Kit; Human Rho-associated coiled-coil containing protein kinase 2 (Rock-2) ELISA Kit; KIAA0619; ; ROCK2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ROCK2. No significant cross-reactivity or interference between Human ROCK2 and analogues was observed.
• Sequence Length: 1388

• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml-40 ng/ml
• Sensitivity: 0.156ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ROCK2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Rock-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Rock-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Rock-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Rock-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 41872583
• NCBI GeneID: 9475
• NCBI Accession #: NP_004841.2
• NCBI GenBank Nucleotide #: NM_004850.3
• UniProt Accession #: O75116; Q53QZ0; Q53SJ7; Q9UQN5
• Molecular Weight: 160,900 Da
• NCBI Official Full Name: rho-associated protein kinase 2
• NCBI Official Synonym Full Names: Rho-associated, coiled-coil containing protein kinase 2
• NCBI Official Symbol: ROCK2
• NCBI Official Synonym Symbols: ROCK-II
• NCBI Protein Information: rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II
• UniProt Protein Name: Rho-associated protein kinase 2
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: ROCK2
• UniProt Synonym Gene Names: KIAA0619; ROCK-II
• UniProt Entry Name: ROCK2_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca2+ sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.8 PublicationsManual assertion based on experiment in:Ref.4

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium

Enzyme regulation: Activated by RHOA binding. Inhibited by Y-27632