Ring Finger Protein 34 Recombinant Protein | RNF34 recombinant protein

Recombinant Human Ring Finger Protein 34

Cat. Num. AAA145565

• Gene Names: RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Ring Finger Protein 34; Recombinant Human Ring Finger Protein 34; RNF34 Human; Ring Finger Protein 34 Human Recombinant; E3 Ubiquitin Protein Ligase; RING Finger Protein 34; Caspase Regulator CARP1; Caspases-8 And -10-Associated RING Finger Protein 1; FYVE-RING Finger Protein Momo; Human RING Finger Homologous To Inhibitor Of Apoptosis Protein; CARP-1; hRFI; RING Finger Protein RIFF; RFI; CARP1; RIF; RIFF; E3 Ubiquitin-Protein Ligase RNF34; EC 6.3.2; RNF34 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: RNF34 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGS MRKAGAT SMWASCCGLL NEVMGTGAVR GQQSAFAGAT GPFRFTPNPEFSTYPPAATE GPNIVCKACG LSFSVFRKKH VCCDCKKDFC SVCSVLQENL RRCSTCHLLQ ETAFQRPQLMRLKVKDLRQY LILRNIPIDT CREKEDLVDL VLCHHGLGSE DDMDTSSLNS SRSQTSSFFT RSFFSNYTAPSATMSSFQGE LMDGDQTSRS GVPAQVQSEI TSANTEDDDD DDDEDDDDEE ENAEDRNPGL SKERVRASLSDLSSLDDVEG MSVRQLKEIL ARNFVNYSGC CEKWELVEKV NRLYKENEEN QKSYGERLQL QDEEDDSLCR ICMDAVIDCVLLECGHMVTC TKCGKRMSEC PICRQYVVRA VHVFKS
• Sequence Length: 180

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

Related Product Information for RNF34 recombinant protein

Description: RNF34 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 396 amino acids (1-373a.a) and having a molecular mass of 44.2kDa.RNF34 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Ring Finger Protein 34 (RNF34) has E3 ubiquitin-protein ligase activity. RNF34 has a RINF finger, a motif recognized to be involved in protein-protein and protein-DNA interactions. RNF34 regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. In addition, RNF34 protects cells against apoptosis induced by TNF. RNF34 also binds phosphatidylinositol 5-phosphateand phosphatidylinositol 3-phosphate. Alternatively splicing results in multiple transcript variants encoding distinct isoforms.

Product Categories/Family for RNF34 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Ring Finger Protein

NCBI and Uniprot Product Information

• NCBI GI #: 378925642
• NCBI GeneID: 80196
• NCBI Accession #: NP_001243787.1
• NCBI GenBank Nucleotide #: NM_001256858.1
• UniProt Accession #: Q969K3; Q8NG47; Q9H6W8; B7Z933
• Molecular Weight: 41,797 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF34 isoform 3
• NCBI Official Synonym Full Names: ring finger protein 34, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF34
• NCBI Official Synonym Symbols: RFI; RIF; RIFF; hRFI; CARP1; CARP-1
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF34; FYVE-RING finger protein MOMO; RING finger protein RIFF; caspase regulator CARP1; caspases-8 and -10-associated RING finger protein 1; human RING finger homologous to inhibitor of apoptosis protein
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF34
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF34
• UniProt Entry Name: RNF34_HUMAN

NCBI Description

The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Uniprot Description

RNF34: Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3- phosphate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cytoplasm; plasma membrane; endomembrane system; nuclear speck; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination