E3 ubiquitin-protein ligase RNF168 (Rnf168) Recombinant Protein | Rnf168 recombinant protein
Recombinant Rat E3 ubiquitin-protein ligase RNF168 (Rnf168)
NCBI and Uniprot Product Information
Uniprot Description
E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).
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Product Notes
The Rnf168 rnf168 (Catalog #AAA1236869) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-564, Full length protein. The amino acid sequence is listed below: MAAPKNSIPS LAECQCGICM EILVEPVTLP CNHTLCNPCF QSTVEKANLC CPFCRRRVSS WTRYHTRRNS LVNTDLWEII QKHYAKECKL RISGQESKEI VDEYQPVRLL SKPGELRREY EEEISKVEAE RQASKEEENK ASEEYIQRLL AEEEEEEKRR TERRRSEMEE QLRGDEELAR RLSTSINSNY ERNILASPLS SRKSDPVTNK SQKKNTNKQK NFGDIQRYLS PKSKPGTAWA CKTEHGEDMC KSKETDSSDT KSPVLQDTDV EESMPTHSPQ TCPETQGQGP EPLTEMPVPW LCARNAEQCL EGKAEAVSTN PDDSCIVNDG GPRAIVSNSK EAAVKPPTKI ENEEYSVSGV TQLTGGNGVP TESRVYDLLV GKEISERENQ ESVFEEVMDP CFSAKRRKIF ITSSLDQEET EVNFTQKLID LEHMLFERHK QEEQDRLLAL QLQKEADKEK MVPNRQKGSP DQYQLRTSSP PDGLLNGQRK NVKDRNSPKQ TADRSKSQRS RKGEYWETFE STWKGSVNGT KMPTPRKDSC NVSKRACPLQ HRSAQKSILQ MFQR. It is sometimes possible for the material contained within the vial of "E3 ubiquitin-protein ligase RNF168 (Rnf168), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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