Typical Testing Data/Standard Curve (for reference only)
Monkey Ribonuclease A ELISA Kit | RNaseA elisa kit
Monkey Ribonuclease A ELISA Kit
Reactivity
Monkey
Synonyms
Ribonuclease A; Monkey Ribonuclease A ELISA Kit; RNaseA elisa kit
Reactivity
Monkey
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RNaseA elisa kit
Intended Uses: This RNaseA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey RNaseA. This ELISA kit for research use only!
Principle of the Assay: RNaseA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RNaseA antibody and an RNaseA-HRP conjugate. The assay sample and buffer are incubated together with RNaseA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RNaseA concentration since RNaseA from samples and RNaseA-HRP conjugate compete for the anti-RNaseA antibody binding site. Since the number of sites is limited, as more sites are occupied by RNaseA from the sample, fewer sites are left to bind RNaseA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNaseA concentration in each sample is interpolated from this standard curve.
Principle of the Assay: RNaseA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RNaseA antibody and an RNaseA-HRP conjugate. The assay sample and buffer are incubated together with RNaseA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RNaseA concentration since RNaseA from samples and RNaseA-HRP conjugate compete for the anti-RNaseA antibody binding site. Since the number of sites is limited, as more sites are occupied by RNaseA from the sample, fewer sites are left to bind RNaseA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNaseA concentration in each sample is interpolated from this standard curve.
Product Categories/Family for RNaseA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
17,644 Da
NCBI Official Full Name
ribonuclease A
UniProt Protein Name
Ribonuclease pancreatic
Protein Family
UniProt Gene Name
RNASE1
UniProt Synonym Gene Names
RIB1; RNS1; RNase 1; RNase A
UniProt Entry Name
RNAS1_HUMAN
Uniprot Description
RNASE1: Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA. Belongs to the pancreatic ribonuclease family.
Protein type: Secreted; EC 3.1.27.5; Secreted, signal peptide; Ribonuclease
Chromosomal Location of Human Ortholog: 14q11.2
Molecular Function: protein binding; nucleic acid binding; pancreatic ribonuclease activity
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Product Notes
The Monkey RNaseA rnase1 (Catalog #AAA741934) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741934 ELISA Kit recognizes Monkey RNaseA. It is sometimes possible for the material contained within the vial of "Ribonuclease A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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