Ribonuclease 1 Recombinant Protein | RNASE1 recombinant protein
Recombinant Human Ribonuclease 1
Gene Names
RNASE1; RAC1; RIB1; RNS1
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
Ribonuclease 1; Recombinant Human Ribonuclease 1; Ribonuclease 1 Human Recombinant; RNASE1; ribonuclease A family member 1; pancreatic; RAC1; RIB1; RNS1; ribonuclease pancreatic; HP-RNase; RIB-1; RNase UpI-1; RNase 1; Ribonuclease A; RNase A; RNASE1 Human; RNASE1 recombinant protein
Host
HEK293 Cells
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Form/Format
The RNASE1 solution (1mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH7.4).
Sterile Filtered colorless solution.
Sterile Filtered colorless solution.
Sequence
KESRAKKFQR QHMDSDSSPS SSSTYCNQMM RRRNMTQGRC KPVNTFVHEP LVDVQNVCFQ EKVTCKNGQG NCYKSNSSMH ITDCRLTNGS RYPNCAYRTS PKERHIIVAC EGSPYVPVHF DASVEDSTHH HHHH
Biological Activity
Specific activity is >3 X 10^6unit/mg. Defined by the amount of enzyme that hydrolyzes 1nmole of RNA per minute at 25 degree C.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Related Product Information for RNASE1 recombinant protein
Introduction: Ribonuclease 1R (NASE1) is a small protein which is a part of pancreatic ribonuclease enzyme family. NASE1 has 4 disulfide bonds in its native state and cleaves speciallyafter pyrimidine nucleotides.Cleavage takes place in 2 steps: first, the 3',5'-phosphodiester bond is cleaved to craete a 2',3'-cyclic phosphodiester intermediate; than, the cyclic phosphodiester is hydrolyzed to a 3'-monophosphate. NASE1is activated the most with single stranded RNA.NASE1inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.NASE1hydrolyzes RNA from protein samples.
Description: RNASE1 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (29-156 a.a) containing a total of 134 amino acids, having a molecular mass of 15.3kDa.
RNASE1 is fused to a 6 amino acid His-tag at C-terminus,and is purified by proprietary chromatographic techniques.
Description: RNASE1 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (29-156 a.a) containing a total of 134 amino acids, having a molecular mass of 15.3kDa.
RNASE1 is fused to a 6 amino acid His-tag at C-terminus,and is purified by proprietary chromatographic techniques.
Product Categories/Family for RNASE1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,644 Da
NCBI Official Full Name
ribonuclease pancreatic
NCBI Official Synonym Full Names
ribonuclease A family member 1, pancreatic
NCBI Official Symbol
RNASE1
NCBI Official Synonym Symbols
RAC1; RIB1; RNS1
NCBI Protein Information
ribonuclease pancreatic
UniProt Protein Name
Ribonuclease pancreatic
Protein Family
UniProt Gene Name
RNASE1
UniProt Synonym Gene Names
RIB1; RNS1; RNase 1; RNase A
NCBI Description
This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.
Research Articles on RNASE1
1. Data suggest that ribonuclease inhibitor (RNH1) protects HeLa cells from ribonuclease 1 (RNase 1).
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Product Notes
The RNASE1 rnase1 (Catalog #AAA147351) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: KESRAKKFQR QHMDSDSSPS SSSTYCNQMM RRRNMTQGRC KPVNTFVHEP LVDVQNVCFQ EKVTCKNGQG NCYKSNSSMH ITDCRLTNGS RYPNCAYRTS PKERHIIVAC EGSPYVPVHF DASVEDSTHH HHHH. It is sometimes possible for the material contained within the vial of "Ribonuclease 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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