Monkey Relaxin ELISA Kit | RLT elisa kit

Monkey Relaxin ELISA Kit

Cat. Num. AAA29016

• Gene Names: RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• Reactivity: Monkey
• Synonyms: Relaxin; Monkey Relaxin ELISA Kit; RLT elisa kit

• Reactivity: Monkey
• Specificity: No significant cross-reactivity or interference between Monkey RLT and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated RLT concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey RLT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RLT and RLT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RLT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for RLT elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated RLT concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey RLT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RLT and RLT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RLT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 490056
• NCBI GeneID: 6013
• UniProt Accession #: P04808; Q99936; Q9UQJ1
• Molecular Weight: 21,146 Da
• NCBI Official Full Name: relaxin
• NCBI Official Synonym Full Names: relaxin 1
• NCBI Official Symbol: RLN1
• NCBI Official Synonym Symbols: H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• NCBI Protein Information: prorelaxin H1; preprorelaxin H1
• UniProt Protein Name: Prorelaxin H1
• Protein Family: Relaxin
• UniProt Gene Name: RLN1
• UniProt Entry Name: REL1_HUMAN

NCBI Description

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The protein encoded by this gene is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. [provided by RefSeq, Jan 2013]

Uniprot Description

RLN1: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: female pregnancy; signal transduction