Western Blot (WB)
(Western blot analysis of D4-GDI with Jurkat cell lysate.)
Rabbit D4GDI Polyclonal Antibody | anti-D4GDI antibody
D4GDI antibody
Applications
Western Blot
Purity
D4GDI antibody was purified by affinity chromatography
Synonyms
D4GDI; Polyclonal Antibody; D4GDI antibody; Polyclonal D4GDI; anti-D4GDI; GDID4; GDIA2; DGDI 4; P52566; DGDI-4; RAP1GN1; Ly-GDI; 602843; ARHGDIB; LYGDI; D4; anti-D4GDI antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
D4GDI antibody was purified by affinity chromatography
Form/Format
Supplied in PBS buffer containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-D4GDI antibody
Western Blot (WB)
Application Notes
WB: 0.5-4 ug/ml
Biological Significance
D4-GDI is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeleytal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kDa and 5kDa size fragment. The 23kDa fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
Cross-Reactivity
Human
Immunogen
D4GDI antibody was raised in Rabbit using synthetic peptide near the N-terminus of human D4-GDI as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of D4-GDI with Jurkat cell lysate.)
Western Blot (WB)
(Western blot analysis of D4-GDI with Jurkat cell lysate.)
Related Product Information for anti-D4GDI antibody
Rabbit polyclonal D4GDI antibody
Product Categories/Family for anti-D4GDI antibody
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Product Notes
The D4GDI (Catalog #AAA837331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's D4GDI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the D4GDI for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "D4GDI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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