Regulator Of G Protein Signaling 6 Recombinant Protein | RGS6 recombinant protein

Recombinant Regulator Of G Protein Signaling 6 (RGS6)

Cat. Num. AAA2033236

• Gene Names: RGS6; GAP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Regulator Of G Protein Signaling 6; Recombinant Regulator Of G Protein Signaling 6 (RGS6); GAP; S914; RGS6 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MAQGSGDQRA VGVADPEESS PNMIVYCKIE DIITKMQDDK TGGVPIRTVK SFLSKIPSVV TGTDIVQWLM KNLSIEDPVE AIHLGSLIAA QGYVFPISDH VLTMKDDGTF YRFQAPYFWP SNCWEPENTD YAIYLCKRTM QNKARLELAD YEAENLARLQ RAFARKWEFI FMQAEAQVKI DRKKDKTERK ILDSQERAFW DVHRPVPGCV NTTEMDIRKC RRLKNPQKVK KSVYGVTDES QSQSPVHIPS QPIRKTTKDD IRKQITFLNA QIDRHCLKMS KVAESLIAYT EQYVEYDPFI TPAEPSNPWI SDDITLWDIE MSKEPSQQRV KRWGFSFDEI LKDQVGRDQF LRFLESEFSS ENLRFWLAVQ DLKKQPLQDV AKRVEEIWQE FLAPGAPSAI NLDSHSYEIT SQNVKDGGRYTFEDAQEHIY KLMKSDSYAR FLRSNAYQDL LLAKKKGKSL AGKRLTGLMQ SS
• Sequence Length: 472

• Applicable Applications for RGS6 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 58.2kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Ser472
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 18057198
• NCBI GeneID: 9628
• Molecular Weight: 53,319 Da
• NCBI Official Full Name: regulator of G-protein signaling 6
• NCBI Official Synonym Full Names: regulator of G-protein signaling 6
• NCBI Official Symbol: RGS6
• NCBI Official Synonym Symbols: GAP
• NCBI Protein Information: regulator of G-protein signaling 6
• UniProt Protein Name: Regulator of G-protein signaling 6
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS6
• UniProt Synonym Gene Names: RGS6
• UniProt Entry Name: RGS6_HUMAN

NCBI Description

This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins, which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP and GGL domains, the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling, and may modulate neuronal, cardiovascular, lymphocytic activities, and cancer risk. Many alternatively spliced transcript variants encoding different isoforms with long or short N-terminal domains, complete or incomplete GGL domains, and distinct C-terminal domains, have been described for this gene, however, the full-length nature of some of these variants is not known.[provided by RefSeq, Mar 2011]

Uniprot Description

RGS6: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(o)-alpha is specifically enhanced by the RGS6/Gbeta5 dimer. 11 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: cytoplasm; cytosol; extrinsic to membrane; heterotrimeric G-protein complex; plasma membrane

Molecular Function: GTPase activator activity; protein binding; signal transducer activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of signal transduction; positive regulation of GTPase activity; regulation of G-protein coupled receptor protein signaling pathway