Regulator of G-Protein Signaling 14 Recombinant Protein | RGS14 recombinant protein

Recombinant Human Regulator of G-Protein Signaling 14

Cat. Num. AAA144949

• Purity: Greater than 80% as determined by SDS-PAGE.
• Synonyms: Regulator of G-Protein Signaling 14; Recombinant Human Regulator of G-Protein Signaling 14; RGS14 Human; Regulator of G-Protein Signaling 14 Human Recombinant; Regulator of G-protein signaling 14; RGS14; RGS14 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 80% as determined by SDS-PAGE.
• Form/Format: RGS14 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer, pH7.5, 10% glycerol, 1mM DTT and 200mM NaCl.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MPGKPKHLGV PNGRMVLAVS DGELSSTTGP QGQGEGRGSS LSIHSLPSGP SSPFPTEEQP VASWALSFER LLQDPLGLAY FTEFLKKEFS AENVTFWKAC ERFQQIPASD TQQLAQEARN IYQEFLSSQA LSPVNIDRQA WLGEEVLAEP RPDMFRAQQL QIFNLMKFDS YARFVKSPLY RECLLAEAEG RPLREPGSSR LGSPDATRKK PKLKPGKSLP LGVEELGQLP PVEGPGGRPL RKSFRRELGG TANAALRRES QGSLNSSASL DLGFLAFVSS KSESHRKSLG STEGESESRP GKYCCVYLPD GTASLALARP GLTIRDMLAG ICEKRGLSLPDIKVYLVGNE QALVLDQDCT VLADQEVRLE NRITFELELT ALERVVRISA KPTKRLQEAL QPILEKHGLS PLEVVLHRPG EKQPLDLGKL VSSVAAQRLV LDTLPGVKIS KARDKSPCRS QGCPPRTQDK ATHPPPASPS SLVKVPSSAT GKRQTCDIEG LVELLNRVQS SGAHDQRGLLRKEDLVLPEF LQLPAQGPSS EETPPQTKSA AQPIGGSLNS TTDSAL.
• Sequence Length: 566

Related Product Information for RGS14 recombinant protein

Description: RGS14 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 586 amino acids (1-566 a.a) and having a molecular mass of 63.6kDa.RGS14 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Regulator of G-protein signaling 14 (RGS14) is a member of the regulator of G protein signaling family. RGS14 contains one RGS domain, 2 Raf-like Ras-binding domains (RBDs) and one GoLoco motif. RGS14 reduces the signaling activity of G-proteins by binding, via its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. RGS14 is a scaffolding protein which integrates G protein and H-Ras/ERK/MAP kinase signaling pathways, thus making it suitably positioned to repress plasticity in CA2 neurons. Since RGS14 is highly enriched in CA2 pyramidal neurons, it has a role in inhibition of both synaptic plasticity at these synapses and hippocampal-based learning and memory.

Product Categories/Family for RGS14 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Regulator of G-Protein Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 21361304
• NCBI GeneID: 10636
• NCBI Accession #: NP_006471.2
• NCBI GenBank Nucleotide #: NM_006480.4
• UniProt Accession #: O43566; O43565; Q506M1; Q6ZWA4; Q8TD62
• Molecular Weight: 36,922 Da
• NCBI Official Full Name: regulator of G-protein signaling 14
• NCBI Official Synonym Full Names: regulator of G-protein signaling 14
• NCBI Official Symbol: RGS14
• NCBI Protein Information: regulator of G-protein signaling 14; regulator of G-protein signalling 14
• UniProt Protein Name: Regulator of G-protein signaling 14
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS14
• UniProt Synonym Gene Names: RGS14
• UniProt Entry Name: RGS14_HUMAN

NCBI Description

This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS14: Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS1 and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Probably required for the nerve growth factor (NGF)-mediated neurite outgrowth. May be involved in visual memory processing capacity and hippocampal-based learning and memory. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: spindle pole; centrosome; microtubule; PML body; postsynaptic density; dendrite; dendritic spine; nuclear body; postsynaptic membrane; cytoplasm; plasma membrane; spindle; cell junction; nucleus

Molecular Function: receptor signaling complex scaffold activity; microtubule binding; receptor signaling protein activity; GDP-dissociation inhibitor activity; G-protein alpha-subunit binding; GTPase activating protein binding; protein kinase binding; GTPase activator activity

Biological Process: regulation of transcription in response to stress; mitosis; zygote asymmetric cell division; negative regulation of MAP kinase activity; positive regulation of neurogenesis; negative regulation of synaptic plasticity; platelet-derived growth factor receptor signaling pathway; nucleocytoplasmic transport; learning; spindle organization and biogenesis; chromosome segregation; long-term memory; cell division; visual learning; response to oxidative stress; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity