Human regulator of G-protein signaling 11 ELISA Kit | RGS11 elisa kit

Human Regulator of G-protein signaling 11, RGS11 ELISA Kit

Cat. Num. AAA9328990

• Gene Names: RGS11; RS11
• Reactivity: Human
• Synonyms: regulator of G-protein signaling 11; Human Regulator of G-protein signaling 11; RGS11 ELISA Kit; Human Regulator of G-protein signaling 11 (RGS11) ELISA kit; AC004754.2; RS11; regulator of G-protein signalling 11; RGS11 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human RGS11 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 3.12ng/ml-100ng/ml.
• Sensitivity: 1.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated RGS11 concentrations in Human serum, plasma and other body fluids. Using Purified Human RGS11 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RGS11 and RGS11 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RGS11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for RGS11 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated RGS11 concentrations in Human serum, plasma and other body fluids. Using Purified Human RGS11 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RGS11 and RGS11 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RGS11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 557129067
• NCBI GeneID: 8786
• NCBI Accession #: NP_001273414.1
• NCBI GenBank Nucleotide #: NM_001286485.1
• UniProt Accession #: O94810; O75883; Q4TT71; Q4TT72
• Molecular Weight: 52,946 Da
• NCBI Official Full Name: regulator of G-protein signaling 11 isoform 3
• NCBI Official Synonym Full Names: regulator of G-protein signaling 11
• NCBI Official Symbol: RGS11
• NCBI Official Synonym Symbols: RS11
• NCBI Protein Information: regulator of G-protein signaling 11; regulator of G-protein signalling 11
• UniProt Protein Name: Regulator of G-protein signaling 11
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS11
• UniProt Synonym Gene Names: RGS11
• UniProt Entry Name: RGS11_HUMAN

NCBI Description

The protein encoded by this gene belongs to the RGS (regulator of G protein signaling) family. Members of the RGS family act as GTPase-activating proteins on the alpha subunits of heterotrimeric, signal-transducing G proteins. This protein inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2013]

Uniprot Description

RGS11: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: protein complex; cytoplasm; plasma membrane; heterotrimeric G-protein complex

Molecular Function: signal transducer activity; G-protein beta-subunit binding; GTPase activator activity

Biological Process: G-protein coupled receptor protein signaling pathway; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity