Typical Testing Data/Standard Curve (for reference only)
Chicken TR ELISA Kit | TR elisa kit
Chicken TR (Testosterone Receptor) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TR. You can calculate the concentration of TR in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
receptor for transferrin; involved in regulating stellate cell activation [RGD, Feb 2006]
Uniprot Description
TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.
Protein type: Receptor, misc.; Cell surface; Membrane protein, integral
Cellular Component: extracellular space; cell surface; mitochondrion; intracellular membrane-bound organelle; cell; recycling endosome membrane; cytoplasmic membrane-bound vesicle; extracellular region; integral to membrane; coated pit; recycling endosome; perinuclear region of cytoplasm; plasma membrane; melanosome; vesicle; endosome; external side of plasma membrane
Molecular Function: identical protein binding; transferrin receptor activity; chaperone binding; double-stranded RNA binding; Hsp70 protein binding
Biological Process: receptor-mediated endocytosis; response to retinoic acid; response to inorganic substance; cellular iron ion homeostasis; transferrin transport; osteoclast differentiation; regulation of cell proliferation; response to organic substance; response to manganese ion; response to copper ion; response to hypoxia; acute-phase response; positive regulation of bone resorption; regulation of cell growth; response to iron ion; response to nutrient; aging
Research Articles on TR
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Product Notes
The Chicken TR tfrc (Catalog #AAA2505205) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2505205 ELISA Kit recognizes Chicken TR. It is sometimes possible for the material contained within the vial of "TR, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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