RET / Proto-Oncogene Tyrosine-Protein Kinase Receptor Ret Recombinant Protein | RET recombinant protein

Human RET / Proto-Oncogene Tyrosine-Protein Kinase Receptor Ret Recombinant Protein

• Gene Names: RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: RET / Proto-Oncogene Tyrosine-Protein Kinase Receptor Ret; Human RET / Proto-Oncogene Tyrosine-Protein Kinase Receptor Ret Recombinant Protein; Proto-oncogene tyrosine-protein kinase receptor Ret; Cadherin family member 12; Proto-oncogene c-Ret; CDHF12; CDHR16; PTC; RET51; RET recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 1072

• Applicable Applications for RET recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Phosphorylated form interacts with the PBT domain of DOK2, DOK4 and DOK5. The phosphorylated form interacts with PLCG1 and GRB7. Interacts (not phosphorylated) with PTK2/FAK1 (via FERM domain). Extracellular cell-membrane anchored RET cadherin fragments form complex in neurons with reduced trophic status, preferentially at the contact sites between somas. Interacts with AIP in the pituitary gland; this interaction prevents the formation of the AIP-survivin complex. Binds to ARTN. Interacts (inactive) with CBLC and CD2AP; dissociates upon activation by GDNF which increases CBLC:CD2AP interaction.
• Entry Function: Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 10862701
• NCBI GeneID: 5979
• NCBI Accession #: NP_065681.1
• NCBI GenBank Nucleotide #: NP_065681.1
• UniProt Accession #: P07949
• NCBI Official Full Name: proto-oncogene tyrosine-protein kinase receptor Ret isoform c
• NCBI Official Synonym Full Names: ret proto-oncogene
• NCBI Official Symbol: RET
• NCBI Official Synonym Symbols: PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase receptor Ret
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase receptor Ret
• Protein Family: Proto-oncogene tyrosine-protein kinase receptor
• UniProt Gene Name: RET
• UniProt Synonym Gene Names: CDHF12; CDHR16; PTC; RET51
• UniProt Entry Name: RET_HUMAN

NCBI Description

This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis. [provided by RefSeq, Sep 2017]

Uniprot Description

Ret: a proto-oncogenic receptor tyrosine kinase. Receptor for glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Part of a multicompetent receptor complex with other membrane-bound ligand-binding GDNF family receptors (aGFRs). Required for development of the kidney and neural crest-derived cell types. Three alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Oncoprotein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; Kinase, protein; TK group; Ret family

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; endosome membrane; receptor complex; lipid raft

Molecular Function: protein binding; protein-tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; calcium ion binding; ATP binding

Biological Process: response to drug; regulation of cell adhesion; caspase activation; positive regulation of neuron maturation; posterior midgut development; peptidyl-tyrosine phosphorylation; positive regulation of cell size; positive regulation of transcription, DNA-dependent; membrane protein proteolysis; MAPKKK cascade; positive regulation of cell adhesion mediated by integrin; response to pain; regulation of axonogenesis; signal transduction; neuron maturation; protein amino acid phosphorylation; enteric nervous system development; retina development in camera-type eye; ureteric bud development; embryonic epithelial tube formation; neuron adhesion; homophilic cell adhesion; neural crest cell migration; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

Disease: Multiple Endocrine Neoplasia, Type Iib; Multiple Endocrine Neoplasia, Type Iia; Thyroid Carcinoma, Papillary; Hirschsprung Disease, Susceptibility To, 1; Thyroid Carcinoma, Familial Medullary