Rabbit Ret Polyclonal Antibody | anti-Ret antibody

Phospho-Ret (Tyr1015) Antibody

Cat. Num. AAA9614202

• Gene Names: RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (88%), Bovine (88%), Sheep (88%), Rabbit (100%), Dog (88%)
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: Ret; Polyclonal Antibody; Phospho-Ret (Tyr1015) Antibody; C ret; Cadherin family member 12; Cadherin related family member 16; CDHF 12; CDHF12; CDHR16; ELKS Fusion gene; HSCR 1; HSCR1; Hydroxyaryl protein kinase; MEN2A; MEN2B; MTC 1; MTC1; Multiple endocrine neoplasia and medullary thyroid carcinoma 1; Oncogene RET; Proto oncogene tyrosine protein kinase receptor ret; Proto-oncogene c-Ret; Proto-oncogene tyrosine-protein kinase receptor ret; PTC; RET; RET ELE1; Ret Proto oncogene; RET transforming sequence; RET_HUMAN; RET51; RET9; tyrosine-protein kinase receptor ret; anti-Ret antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (88%), Bovine (88%), Sheep (88%), Rabbit (100%), Dog (88%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-Ret (Tyr1015) Antibody detects endogenous levels of Ret only when phosphorylated at Tyr1015.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Ret antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Ret around the phosphorylation site of Tyr1015.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Dephosphorylated by PTPRJ on Tyr-905, Tyr-1015 and Tyr-1062.Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons.
• Subunit Structure: Phosphorylated form interacts with the PBT domain of DOK2, DOK4 and DOK5 (By similarity). The phosphorylated form interacts with PLCG1 and GRB7 (By similarity). Interacts (not phosphorylated) with PTK2/FAK1 (via FERM domain). Extracellular cell-membrane anchored RET cadherin fragments form complex in neurons with reduced trophic status, preferentially at the contact sites between somas. Interacts with AIP in the pituitary gland; this interaction prevents the formation of the AIP-survivin complex. Binds to ARTN (By similarity). Interacts (inactive) with CBLC and CD2AP; dissociates upon activation by GDNF which increases CBLC:CD2AP interaction. Interacts (via the extracellular domain) with GFRAL (via the extracellular domain); the interaction mediates cellular signaling upon interaction of GFRAL with its ligand GDF15. Interaction with GFRAL requires previous GDF15-binding to GFRAL. Interacts with GFRA1; in the presence of SORL1, the GFRA1/RET complex is targeted to endosomes. Interacts with GDNF.
• Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family.
• Subcellular Location: Cell membrane>Single-pass type I membrane protein. Endosome membrane>Single-pass type I membrane protein.; Note: Predominantly located on the plasma membrane. In the presence of SORL1 and GFRA1, directed to endosomes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of c-Ret (Phospho-Tyr1015) using EGF treated A431 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Immunohistochemistry (IHC)

(Staining Rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

Testing Data

(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

Related Product Information for anti-Ret antibody

Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which induces inhibition of food-intake. Activates MAPK- and AKT-signaling pathways. Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL.

NCBI and Uniprot Product Information

• NCBI GI #: 10862701
• NCBI GeneID: 5979
• NCBI Accession #: NP_065681.1
• NCBI GenBank Nucleotide #: NM_020630.4
• UniProt Accession #: P07949; Q15250; Q9BTB0; Q9H4A2; A8K6Z2
• Molecular Weight: 124,319 Da
• NCBI Official Full Name: proto-oncogene tyrosine-protein kinase receptor Ret isoform c
• NCBI Official Synonym Full Names: ret proto-oncogene
• NCBI Official Symbol: RET
• NCBI Official Synonym Symbols: PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase receptor Ret; proto-oncogene c-Ret; receptor tyrosine kinase; RET transforming sequence; cadherin family member 12; hydroxyaryl-protein kinase; cadherin-related family member 16; ret proto-oncogene (multiple endocrin
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase receptor Ret
• Protein Family: Retbindin
• UniProt Gene Name: RET
• UniProt Synonym Gene Names: CDHF12; CDHR16; PTC; RET51
• UniProt Entry Name: RET_HUMAN

NCBI Description

This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008]

Uniprot Description

Ret: a proto-oncogenic receptor tyrosine kinase. Receptor for glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Part of a multicompetent receptor complex with other membrane-bound ligand-binding GDNF family receptors (aGFRs). Required for development of the kidney and neural crest-derived cell types. Three alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Oncoprotein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; Kinase, protein; TK group; Ret family

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; endosome membrane; receptor complex; lipid raft

Molecular Function: protein binding; protein-tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; calcium ion binding; ATP binding

Biological Process: response to drug; regulation of cell adhesion; caspase activation; positive regulation of neuron maturation; posterior midgut development; peptidyl-tyrosine phosphorylation; positive regulation of cell size; positive regulation of transcription, DNA-dependent; membrane protein proteolysis; MAPKKK cascade; positive regulation of cell adhesion mediated by integrin; response to pain; regulation of axonogenesis; signal transduction; neuron maturation; protein amino acid phosphorylation; enteric nervous system development; retina development in camera-type eye; ureteric bud development; embryonic epithelial tube formation; neuron adhesion; homophilic cell adhesion; neural crest cell migration; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

Disease: Multiple Endocrine Neoplasia, Type Iib; Multiple Endocrine Neoplasia, Type Iia; Thyroid Carcinoma, Papillary; Hirschsprung Disease, Susceptibility To, 1; Thyroid Carcinoma, Familial Medullary