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SDS-PAGE

ATP-dependent DNA helicase recQ Recombinant Protein | recQ recombinant protein

Recombinant E Coli ATP-dependent DNA helicase recQ

Gene Names
recQ; ECK3816; JW5855
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
ATP-dependent DNA helicase recQ; Recombinant E Coli ATP-dependent DNA helicase recQ; recQ recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid containing glycerol
Sequence Positions
2-606. Partial
Sequence
AQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRPEYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGD
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

SDS-PAGE
Related Product Information for recQ recombinant protein
Involved in the RecF recombination pathway; its gene expression is under the regulation of the SOS system. It is a DNA helicase.
References
The recQ gene of Escherichia coli K12 primary structure and evidence for SOS regulation.Irino N., Nakayama K., Nakayama H.Mol. Gen. Genet. 205:298-304(1986) Analysis of the Escherichia coli genome DNA sequence of the region from 84.5 to 86.5 minutes.Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.Science 257:771-778(1992) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Escherichia coli K-12 a cooperatively developed annotation snapshot -- 2005.Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.Nucleic Acids Res. 34:1-9(2006) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Escherichia coli RecQ protein is a DNA helicase.Umezu K., Nakayama K., Nakayama H.Proc. Natl. Acad. Sci. U.S.A. 87:5363-5367(1990)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
71.9 kDa
NCBI Official Full Name
MULTISPECIES: ATP-dependent DNA helicase RecQ
NCBI Official Symbol
recQ
NCBI Official Synonym Symbols
ECK3816; JW5855
NCBI Protein Information
ATP-dependent DNA helicase
UniProt Protein Name
ATP-dependent DNA helicase RecQ
UniProt Gene Name
recQ
UniProt Entry Name
RECQ_ECOLI

NCBI Description

SSB binds to RecQ and stimulates DNA unwinding (Shereda, 2007). [More information is available at EcoGene: EG10833]. A component of the RecF recombinational pathway, RecQ is a helicase that functions in blocking illegitimate recombination, enhancing topoisomerase activity, initiating SOS signaling and clearing blocked replication forks. [More information is available at EcoCyc: EG10833].

Uniprot Description

Involved in the RecF recombination pathway; its gene expression is under the regulation of the SOS system. It is a DNA helicase.

Research Articles on recQ

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Product Notes

The recQ recq (Catalog #AAA1265156) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-606. Partial. The amino acid sequence is listed below: AQAEVLNLES GAKQVLQETF GYQQFRPGQE EIIDTVLSGR DCLVVMPTGG GKSLCYQIPA LLLNGLTVVV SPLISLMKDQ VDQLQANGVA AACLNSTQTR EQQLEVMTGC RTGQIRLLYI APERLMLDNF LEHLAHWNPV LLAVDEAHCI SQWGHDFRPE YAALGQLRQR FPTLPFMALT ATADDTTRQD IVRLLGLNDP LIQISSFDRP NIRYMLMEKF KPLDQLMRYV QEQRGKSGII YCNSRAKVED TAARLQSKGI SAAAYHAGLE NNVRADVQEK FQRDDLQIVV ATVAFGMGIN KPNVRFVVHF DIPRNIESYY QETGRAGRDG LPAEAMLFYD PADMAWLRRC LEEKPQGQLQ DIERHKLNAM GAFAEAQTCR RLVLLNYFGE GRQEPCGNCD ICLDPPKQYD GSTDAQIALS TIGRVNQRFG MGYVVEVIRG ANNQRIRDYG HDKLKVYGMG RDKSHEHWVS VIRQLIHLGL VTQNIAQHSA LQLTEAARPV LRGESSLQLA VPRIVALKPK AMQKSFGGNY DRKLFAKLRK LRKSIADESN VPPYVVFNDA TLIEMAEQMP ITASEMLSVN GVGMRKLERF GKPFMALIRA HVDGD . It is sometimes possible for the material contained within the vial of "ATP-dependent DNA helicase recQ, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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