SDS-PAGE
ATP-dependent DNA helicase PcrA Recombinant Protein | pcrA recombinant protein
Recombinant Geobacillus stearothermophilus ATP-dependent DNA helicase PcrA
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
ATP-dependent DNA helicase PcrA; Recombinant Geobacillus stearothermophilus ATP-dependent DNA helicase PcrA; Geobacillus stearothermophilus (Bacillus stearothermophilus); pcrA recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
10-289. Partial
Sequence
AHLNKEQQEAVRTTEGPLLIMAGAGSGKTRVLTHRIAYLMAEKHVAPWNILAITFTNKAAREMRERVQSLLGGAAEDVWISTFHSMCVRILRRDIDRIGINRNFSILDPTDQLSVMKTILKEKNIDPKKFEPRTILGTISAAKNELLPPEQFAKRASTYYEKVVSDVYQEYQQRLLRNHSLDFDDLIMTTIQLFDRVPDVLHYYQYKFQYIHIDEYQDTNRAQYTLVKKLAERFQNICAVGDADQSIYRWRGADIQNILSFERDYPNAKVILLEQNYRST
Species
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for pcrA recombinant protein
DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.
References
"Characterisation of Bacillus stearothermophilus PcrA helicase: evidence against an active rolling mechanism." Bird L.E., Brannigan J.A., Subramanya H.S., Wigley D.B. Nucleic Acids Res. 26:2686-2693(1998)
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
34.6 kDa
NCBI Official Full Name
ATP-dependent DNA helicase PcrA
UniProt Protein Name
ATP-dependent DNA helicase PcrA
Protein Family
UniProt Gene Name
pcrA
UniProt Entry Name
PCRA_GEOSE
Uniprot Description
DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.
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Product Notes
The pcrA pcra (Catalog #AAA1006903) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 10-289. Partial. The amino acid sequence is listed below: AHLNKEQQEA VRTTEGPLLI MAGAGSGKTR VLTHRIAYLM AEKHVAPWNI LAITFTNKAA REMRERVQSL LGGAAEDVWI STFHSMCVRI LRRDIDRIGI NRNFSILDPT DQLSVMKTIL KEKNIDPKKF EPRTILGTIS AAKNELLPPE QFAKRASTYY EKVVSDVYQE YQQRLLRNHS LDFDDLIMTT IQLFDRVPDV LHYYQYKFQY IHIDEYQDTN RAQYTLVKKL AERFQNICAV GDADQSIYRW RGADIQNILS FERDYPNAKV ILLEQNYRST . It is sometimes possible for the material contained within the vial of "ATP-dependent DNA helicase PcrA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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