Tryptase, beta1 Active Protein
Tryptase, beta1, Recombinant, Mouse (TPSB1, MCPT7, Mast Cell Protease 7)
Reactivity
Mouse
Purity
Highly Purified95%, as determined by SDS-PAGE and visualized by silver stain.
Synonyms
Tryptase; beta1; Recombinant; Mouse (TPSB1; MCPT7; Mast Cell Protease 7); beta1 active protein
Host
Recombinant mouse
Reactivity
Mouse
Purity/Purification
Highly Purified
95%, as determined by SDS-PAGE and visualized by silver stain.
95%, as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied in a 0.2um sterile-filtered solution of 25mM Tris, 10mM CaCl2, 0.15M sodium chloride, pH 7.5.
Activity
Measured by its ability to cleave a peptide substrate, Benzyloxcarbonyl-Lys-Thiobenzyl ester (Z-K-SBzl), in the presence of 5,5'-Dithio-bis (1-nitrobenzoic acid) (DTNB), as measured by
absorbance at 405nm and using the extinction coefficient of 13,260 M-1cm-1.2. The specific activity, measured with 100uM Z-K-SBzl and 500ng enzyme in 100ul of 50mM MES, pH 6.5 containing 0.1mM DTNB atRT, is 200pmoles/min/ug.
absorbance at 405nm and using the extinction coefficient of 13,260 M-1cm-1.2. The specific activity, measured with 100uM Z-K-SBzl and 500ng enzyme in 100ul of 50mM MES, pH 6.5 containing 0.1mM DTNB atRT, is 200pmoles/min/ug.
Endotoxin Level
1EU/1ug of the protein as determined by the LAL method.
Preparation and Storage
Stable for up to twelve months at -70 degree C from the date of receipt. The protein can be aliquoted and stored at -20 degree to -70 degree C in a manual defrost freezer for six months without a significant loss of activity. Avoid repeated freeze-thaw cycles.
Tryptase beta-1 (Mast Cell Protease 7) is a serine protease with trypsin-like activity. It is stored in the secretory granules of mouse mast cells. It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma. The deduced amino acid sequence consists of a signal peptide (residues 1-18), a pro region (residues 19-28) and the mature chain (residue 29-273). The mature chain was expressed and purified.
Tryptase beta-1 (Mast Cell Protease 7) is a serine protease with trypsin-like activity. It is stored in the secretory granules of mouse mast cells. It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma. The deduced amino acid sequence consists of a signal peptide (residues 1-18), a pro region (residues 19-28) and the mature chain (residue 29-273). The mature chain was expressed and purified.
Related Product Information for Tryptase, beta1 active protein
Source: Human CD33 Signal Peptide (Met 1- Ala 16) Mouse Tryptase beta-1 (Ile 29-Phe 273)
Product Categories/Family for Tryptase, beta1 active protein
Similar Products
Product Notes
The Tryptase, beta1 (Catalog #AAA636372) is an Active Protein produced from Recombinant mouse and is intended for research purposes only. The product is available for immediate purchase. The Tryptase, beta1, Recombinant, Mouse (TPSB1, MCPT7, Mast Cell Protease 7) reacts with Mouse and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Tryptase, beta1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
