KIRREL2 active protein
Kirrel2, Recombinant, Mouse (Kin of Irregular Chiasm-1-Like 2, Nephrin-like 3, NEPH, Nephrin-like Gene 1, NLG1)
Gene Names
KIRREL2; NLG1; NEPH3; FILTRIN
Purity
Highly Purified90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
Kirrel2; Recombinant; Mouse (Kin of Irregular Chiasm-1-Like 2; Nephrin-like 3; NEPH; Nephrin-like Gene 1; NLG1); KIRREL2 active protein
Host
Recombinant Mouse
Purity/Purification
Highly Purified
90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.
Activity
Measured by the ability of the immobilized protein to support the adhesion of MS-1 cells (mouse pancreatic islet endothelial cell line).
Endotoxin Level
1EU/1ug.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for KIRREL2 active protein
Kirrel2 (kin of irregular chiasm-1-like 2), also known as filtrin, NEPH3 (nephrin-like 3) or NLG1 (nephrin-like gene 1), is a 107kD type I transmembrane adhesion protein belonging to the immunoglobulin superfamily.1, 2 Kirrel2 is one of several proteins expressed both in pancreatic islet b cells and kidney glomerulus, and is also found in brain and lymph nodes.1-9 Mouse Kirrel2 cDNA encodes a 700 aa protein that contains a 16 aa signal sequence, a 491 aa extracellular domain with five C2-type Ig-like domains, a 21 aa transmembrane sequence and a 172 aa intracellular domain with a nephrin-like, conserved podocin binding site (mouse aa 589-598).4, 5 An extracellular RGD sequence allows for cell attachment.4 The extracellular domain of mouse Kirrel2 shares 96%, 86%, 85% and 85% amino acid (aa) identity with rat, human, equine and bovine Kirrel2, respectively. It shares 35-37% aa identity with other mouse Kirrel proteins. One mouse isoform that has been described is missing the transmembrane domain (aa 501-533), while another encodes only portions of Ig domains 1-4.5 Human Kirrel2 is downregulated in glomeruli of patients with proteinuria, suggesting an involvement in the maintenance of the glomerular filtration barrier.6 Autoantibodies to Kirrel2 have been identified in a subset of humans with type I diabetes, indicating its involvement with islet b cell function.7 In the brain, Kirrel2 is present in olfactory neurons; but only when they are active; homophilic adhesion of Kirrel2 is postulated to help correlate nerves identifying the same odorant.8 It is also a marker for mouse early postmitotic neural precursors in the ventricular zone of the developing mouse spinal cord.9
A DNA sequence encoding the extracellular domain of mouse Kirrel2 (Met 1-Asp 501; Accession # NP_766486) was fused to a ten-histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of 52.7kD. As a result of glycosylation, the recombinant protein migrates as an ~ 66kD protein in SDS-PAGE under reducing conditions.
A DNA sequence encoding the extracellular domain of mouse Kirrel2 (Met 1-Asp 501; Accession # NP_766486) was fused to a ten-histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of 52.7kD. As a result of glycosylation, the recombinant protein migrates as an ~ 66kD protein in SDS-PAGE under reducing conditions.
Product Categories/Family for KIRREL2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,092 Da
NCBI Official Full Name
kin of IRRE-like protein 2 isoform c
NCBI Official Synonym Full Names
kin of IRRE like 2 (Drosophila)
NCBI Official Symbol
KIRREL2
NCBI Official Synonym Symbols
NLG1; NEPH3; FILTRIN
NCBI Protein Information
kin of IRRE-like protein 2; nephrin-like 3; nephrin-like gene 1; nephrin-like protein 3; kin of irregular chiasm-like 2; kin of irregular chiasm-like protein 2
UniProt Protein Name
Kin of IRRE-like protein 2
Protein Family
UniProt Gene Name
KIRREL2
UniProt Synonym Gene Names
NEPH3
UniProt Entry Name
KIRR2_HUMAN
Uniprot Description
KIRREL2: Belongs to the immunoglobulin superfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.12
Cellular Component: plasma membrane; integral to membrane
Biological Process: cell-cell adhesion; negative regulation of protein amino acid phosphorylation; cell adhesion
Research Articles on KIRREL2
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Product Notes
The KIRREL2 kirrel2 (Catalog #AAA637610) is an Active Protein produced from Recombinant Mouse and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Kirrel2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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