Glypican 1 Active Protein | GPC1 active protein

Glypican 1, Recombinant, Mouse (Glypiated Proteoglycan 1)

Cat. Num. AAA637259

• Gene Names: GPC1; glypican
• Purity: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Glypican 1; Recombinant; Mouse (Glypiated Proteoglycan 1); GPC1 active protein

• Host: Recombinant Mouse
• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.

• Activity: Measured by its ability to bind FGF-basic in a functional ELISA.
• Endotoxin Level: 1EU/1ug.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for GPC1 active protein

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that play a key role in growth factor signaling (1, 2, 3, 4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60-70kD, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known. One subfamily contains Glypicans 1, 2, 4 and 6, while another subfamily contains Glypicans 3 and 5 (3, 5). Mouse Glypican 1 (GPC-1) is synthesized as a 557 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 506 aa mature segment, and a 28 aa C-terminal prosegment (6, 7). There are two potential N-linked, and four potential O-linked sites for glycosylation or glycanation. There is at least one heparan sulfate (HS) modification on GPC-1 that contributes to a native molecular weight of approximately 110kD (8, 9). Mature mouse GPC-1 shares 91% and 98% aa identity with mature human and rat GPC-1, respectively. There are two potential splice variants of mouse GPC-1. The first is truncated and shows a seven amino acid substitution for the first 294 amino acids; the second reveals an alternate start site at Met73 (10, 11). Cells known to express GPC-1 include neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells (8, 9, 12-15). The function of GPC-1 is complex and varied. As a proteoglycan, it appears to make use of its HS adduct to impact select growth factor activity (16). GPC-1 accomplishes its co-receptor role by having juxtramembrane HS attachment sites and a flexible, GPI-linkage (17). Recent data suggests GPC-1 and sulfation enzymes may collaborate to regulate FGF signaling. HS modules that are rich in 2-O- and 6-O- sulfate upregulate FGF-2 activation of FGFR1c (18). Similarly, FGF-1 requires both 2-O- and 6-O-sulfation to bind to FGFR2c and 3c. By contrast, FGF-1 requires no sulfation to bind to FGFR2b, and FGF-8b needs only 6-O-sulfation to activate FGFR3c. Thus, many FGF receptor isoform specific effects may be attributed to an interaction between Glypican family members and the cell sulfation system (19).

A DNA sequence encoding the mature mouse Glypican 1 (Asp 24-Ser 529; Accession # Q9QZF2) was fused to a six-hisidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of 56.4kD. As a result of glycosylation, the recombinant protein migrates as an ~ 57-62kD protein in SDS-PAGE under reducing conditions.

Product Categories/Family for GPC1 active protein

Molecular Biology; MB-Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 167001141
• NCBI GeneID: 2817
• NCBI Accession #: NP_002072.2
• NCBI GenBank Nucleotide #: NM_002081.2
• UniProt Accession #: P35052; Q53QM4
• Molecular Weight: 61,680 Da
• NCBI Official Full Name: glypican-1
• NCBI Official Synonym Full Names: glypican 1
• NCBI Official Symbol: GPC1
• NCBI Official Synonym Symbols: glypican
• NCBI Protein Information: glypican-1; glypican proteoglycan 1
• UniProt Protein Name: Glypican-1
• Protein Family: Glypican
• UniProt Gene Name: GPC1
• UniProt Entry Name: GPC1_HUMAN

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

GPC1: Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Associates (via the heparan sulfate side chains) with fibrillar APP-beta amyloid peptides in primitive and classic amyloid plaques and may be involved in the deposition of these senile plaques in the Alzheimer disease (AD) brain. Misprocessing of GPC1 is found in fibroblasts of patients with Niemann-Pick Type C1 disease. This is due to the defective deaminative degradation of heparan sulfate chains. Belongs to the glypican family.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Extracellular matrix

Chromosomal Location of Human Ortholog: 2q35-q37

Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; extracellular space; Golgi lumen; integral to plasma membrane; plasma membrane; endosome; lipid raft

Molecular Function: heparan sulfate proteoglycan binding; fibroblast growth factor binding; copper ion binding; laminin binding

Biological Process: axon guidance; phototransduction, visible light; heparan sulfate proteoglycan catabolic process; glycosaminoglycan metabolic process; negative regulation of fibroblast growth factor receptor signaling pathway; pathogenesis; Schwann cell differentiation; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; myelin formation; glycosaminoglycan catabolic process; carbohydrate metabolic process; retinoid metabolic process