Dentin Matrix Protein 1 Active Protein | Dmp1 active protein

Dentin Matrix Protein 1, Recombinant, Mouse (DMP1)

Cat. Num. AAA636866

• Gene Names: Dmp1; DENTMAT
• Purity: Purified; 85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Dentin Matrix Protein 1; Recombinant; Mouse (DMP1); Dmp1 active protein

• Host: Recombinant Mouse
• Purity/Purification: Purified; 85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.

• Activity: Measured by its ability to bind rhIntegrin aVb3.
• Endotoxin Level: 1EU/1ug.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Dmp1 active protein

Dentin matrix protein 1 (DMP-1) is a member of the SIBLING family of proteins that includes bone sialoprotein, dentin sialophosphoprotein, MEPE, and osteopontin. These are highly phosphorylated integrin-binding proteins that are rich in acidic amino acids and function in the formation of calcified bone and tooth matrix (1, 2). Its phosphate content, spacing of acidic residues, and calcium-dependent dimerization of DMP-1 contribute to its ability to sequester calcium phosphate clusters and promote hydroxyapatite (HA) crystal formation (3-5). Mature mouse DMP-1 is 487 amino acids (aa) in length. It contains a poly-Pro segment (aa 41-44) and an RGD binding motif (aa 350-352). DMP-1 may be cleaved by BMP-1 family proteases at a single site which is conserved in human, generating a 37kD N-terminal (aa 17-212) and a 57kD C-terminal (aa 213-503) fragment (6). The N-terminal fragment in rat carries chondroitin sulfate (7). The C-terminal fragment alone can nucleate HA crystals, while crystal growth into a needle-like morphology is inhibited by the N-terminal fragment (3, 4). Crystal maturation is dependent on the presence of type I collagen (4). DMP-1 is required for odontoblast differentiation as well as dentin formation (8). Nonphosphorylated DMP-1 is retained intracellularly where it is targeted to the nucleus. Here, it activates the transcription of odontoblast and osteoblast specific genes (9, 10). Early in osteoblast maturation, nuclear DMP-1 is extensively phosphorylated by casein kinase II, triggering its secretion (9). DMP-1 mutations in humans are associated with hypophosphatemia and FGF23 overexpression (11, 12). DMP-1 induces the activation of proMMP-9 and displaces mature MMP-9 from TIMP1 (13). DMP-1 tethers MMP-9 to the cell surface via CD44 and integrins aVb3 and aVb5, promoting tumor cell invasiveness in vitro (14). Full length DMP-1 circulates in human serum in a tight complex with complement factor H (13, 14). When first bound to CD44 or integrin aVb3, DMP-1 can anchor factor H to the cell surface and protect the cell from complement-mediated lysis (15). Mature mouse DMP-1 shares 63%, 61%, and 87% aa sequence identity with bovine, human, and rat DMP-1, respectively.

A DNA sequence encoding the mature mouse DMP-1 (Leu 17-Tyr 503; Accession # AAI13754) fused with 6X Histidines at the C-terminus was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of 52.9kD. The recombinant protein migrates anomalously in SDS-PAGE under reducing conditions as a 57kD monomer, a non-disulfide linked dimer of 99kD, and a tetramer of 180kD.

Product Categories/Family for Dmp1 active protein

Molecular Biology; MB-Extracellular Matrix Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 148540125
• NCBI GeneID: 25312
• NCBI Accession #: NP_987089.2
• NCBI GenBank Nucleotide #: NM_203493.3
• UniProt Accession #: P98193
• Molecular Weight: 53,058 Da
• NCBI Official Full Name: dentin matrix acidic phosphoprotein 1
• NCBI Official Synonym Full Names: dentin matrix acidic phosphoprotein 1
• NCBI Official Symbol: Dmp1
• NCBI Official Synonym Symbols: DENTMAT
• NCBI Protein Information: dentin matrix acidic phosphoprotein 1; AG1; DMP-1; dentin matrix protein 1
• UniProt Protein Name: Dentin matrix acidic phosphoprotein 1
• Protein Family: Dentin matrix acidic phosphoprotein
• UniProt Gene Name: Dmp1
• UniProt Synonym Gene Names: DMP-1
• UniProt Entry Name: DMP1_RAT

NCBI Description

acidic protein associated with the dentin matrix; may be a matrix-associated acidic phosphoprotein; may have a regulatory role in dentin mineralization [RGD, Feb 2006]

Uniprot Description

DMP1: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. Defects in DMP1 are the cause of rickets hypophosphatemic autosomal recessive type 1 (ARHR1). A hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: nucleoplasm; proteinaceous extracellular matrix; intracellular membrane-bound organelle; cytoplasm; nucleolus; nucleus

Molecular Function: extracellular matrix binding

Biological Process: extracellular matrix organization and biogenesis; ossification; biomineral formation; regulation of bone mineralization