Brain-Derived Neurotrophic Factor Active Protein | BDNF active protein

Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)

Cat. Num. AAA650454

• Gene Names: BDNF; MGC34632
• Purity: Highly Purified; 96% by RP-HPLC or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: Brain-Derived Neurotrophic Factor; Recombinant; Human (BDNF); BDNF active protein

• Host: Recombinant, Human (E Coli)
• Purity/Purification: Highly Purified; 96% by RP-HPLC or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a lyophilized powder from 20mM sodium citrate buffer, pH 5. Reconstitute with sterile ddH2O to 100ug/ml, which can then be further diluted to other aqueous solutions. Add 0.1% HSA or BSA for long term storage.

• Biological Activity: Recombinant BDNF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50ng/ml.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a standard solution of BDNF as a Reference Standard.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile ddH2O. For long term it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for BDNF active protein

BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3). Polyclonal antibodies raised against murine NGF have been shown to crossreact with both NT-3 and BDNF.

Product Categories/Family for BDNF active protein

Growth Factors; Cytokines; Growth Factors-BDNF

NCBI and Uniprot Product Information

• NCBI GI #: 179407
• NCBI GeneID: 627
• UniProt Accession #: P23560; Q598Q1; Q6DN19; Q6YNR2; Q6YNR3; Q9BYY7; Q9UC24; A7LA85; A7LA92; D3DQZ2; Q304R1; Q6T2C9; Q6YNR1; Q969N8; Q96TC2
• Molecular Weight: 27,818 Da
• NCBI Official Full Name: brain-derived neurotrophic factor
• NCBI Official Synonym Full Names: brain-derived neurotrophic factor
• NCBI Official Symbol: BDNF
• NCBI Official Synonym Symbols: MGC34632
• NCBI Protein Information: brain-derived neurotrophic factor; abrineurin; neurotrophin; OTTHUMP00000232005; OTTHUMP00000232006; OTTHUMP00000232013; OTTHUMP00000232014; OTTHUMP00000232015; OTTHUMP00000232016; OTTHUMP00000232018; OTTHUMP00000232019; OTTHUMP00000232020; OTTHUMP00000232021; OTTHUMP00000232022; OTTHUMP00000232039; OTTHUMP00000232040; OTTHUMP00000232041; OTTHUMP00000232043
• UniProt Protein Name: Brain-derived neurotrophic factor
• Protein Family: Brain-derived neurotrophic factor
• UniProt Gene Name: BDNF
• UniProt Entry Name: BDNF_HUMAN

NCBI Description

The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq]

Uniprot Description

Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Ref.26

Subunit structure: Monomers and homodimers. Binds to NTRK2/TRKB.

Subcellular location: Secreted.

Tissue specificity: Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. Ref.5

Post-translational modification: The propeptide is N-glycosylated and glycosulfated.Converted into mature BDNF by plasmin (PLG)

Polymorphism: Variations in BDNF are associated with susceptibility to bulimia nervosa 2 (BULN2) [

MIM:610269]. Several genes with an essential role in the regulation of eating behavior and body weight are considered candidates involved in the etiology of eating disorders (ED), but no relevant susceptibility genes with a major effect on anorexia nervosa (AN) or bulimia nervosa (BN) have been identified. BDNF has been implicated in the regulation of food intake and body weight in rodents. A strong association has been reported of the Met-66 allele of the Val-66-Met BDNF variant with restricting AN (ANR) and low minimum body mass index in Spanish patients. Met-66 variant is strongly associated to all ED subtypes (AN, ANR, binge-eating/purging AN and BN) in European patients. Another single nucleotide polymorphism located in the promoter region of the BDNF gene showed an effect on BN and late age at onset of weight loss. These are two variants associated with the pathophysiology of ED in different populations. These variants support a role for BDNF in the susceptibility to aberrant eating behaviors.

Involvement in disease: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [

MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Ref.24

Sequence similarities: Belongs to the NGF-beta family.