GST T1-1 active protein

GST T1-1, Recombinant Human

Cat. Num. AAA483026

• Purity: >= 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
• Synonyms: GST T1-1; Recombinant Human; Recombinant Human GST T1-1; GST T1-1 active protein

• Host: Recombinant 6x His-tagged protein expressed in E Coli
• Purity/Purification: >= 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
• Concentration: 1.95 mg/mL total protein using the Bradford protein assay with BSA as a standard. (varies by lot)
• Sequence Length: 240

• Storage Buffer: 50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT, 1 mM EDTA and 50% glycerol
• Procedural Notes: This purified product exhibits moderate enzymatic activity for Cumene hydroperoxide, the substrate that is most commonly used to assess the glutathione peroxidase activity of soluble GSTs. However, as is suspected for GST activity analysis using synthetic and natural substrates, low concentrations (high dilutions) of the enzyme may result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. Therefore, activity towards GST substrates and these considerations provide guidance when assaying this product under low concentrations or when using substrates other than Cumene hydroperoxide
• Specific Activity: 8.8 Units/mg as assayed by the spectrophotometric determination of NADPH oxidation coupled to the glutathione peroxidase activity of GST T1-1 on cumene hydroperoxide (1.5 mM) in the presence of reduced glutathione (1 mM) in 100 mM NaPO4 (pH 7.0) at room temperature.
• Preparation and Storage: At -20 degree C; AVOID MULTIPLE FREEZE-THAW CYCLES

References

Pemble S., et al., Biochem. J., 300 (Pt 1):271-276 (1994)

NCBI and Uniprot Product Information

• NCBI GI #: 16923707
• NCBI GeneID: 2952
• UniProt Accession #: P30711; O00226; Q5TZY2; Q6IC69; Q969K8; Q96IY3
• Molecular Weight: ~25 kDa
• NCBI Official Full Name: glutathione transferase T1-1
• NCBI Official Synonym Full Names: glutathione S-transferase theta 1
• NCBI Official Symbol: GSTT1
• NCBI Protein Information: glutathione S-transferase theta-1; GST class-theta-1; glutathione transferase T1-1
• UniProt Protein Name: Glutathione S-transferase theta-1
• UniProt Gene Name: GSTT1
• UniProt Entry Name: GSTT1_HUMAN

NCBI Description

The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. GSTT1 and GSTT2 share 55% amino acid sequence identity and both may play an important role in human carcinogenesis. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. This GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes, which are also located on chromosome 22, have been identified. [provided by RefSeq, Jun 2014]

Uniprot Description

GSTT1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Belongs to the GST superfamily. Theta family.

Protein type: Transferase; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cytosol

Molecular Function: glutathione transferase activity; glutathione peroxidase activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process