Exodeoxyribonuclease V gamma chain (recC) Recombinant Protein | recC recombinant protein
Recombinant Escherichia coli Exodeoxyribonuclease V gamma chain (recC) , partial
NCBI and Uniprot Product Information
NCBI Description
RecBCD is a bipolar helicase. [More information is available at EcoGene: EG10825]. Exodeoxyribonuclease V gamma chain. [More information is available at EcoCyc: EG10825].
Uniprot Description
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a rapid (>1 kb/second) and highly processive (>30 kb) ATP-dependent bidirectional helicase. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator, 5'-GCTGGTGG-3') sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to Chi site, by nicking one strand or switching the strand degraded (depending on the reaction conditions). The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang which facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination (PubMed:4562392, PubMed:4552016, PubMed:123277). In the holoenzyme this subunit almost certainly recognizes the wild-type Chi sequence, when added to isolated RecB increases its ATP-dependent helicase processivity. The RecBC complex requires the RecD subunit for nuclease activity, but can translocate along ssDNA in both directions.
Research Articles on recC
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Product Notes
The recC recc (Catalog #AAA1147480) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 805-1122, Fragment at the C-terminal. The amino acid sequence is listed below: EWLPAASQAG KAHSEFVQPL PFTLPETVPL ETLQRFWAHP VRAFFQMRLQ VNFRTEDSEI PDTEPFILEG LSRYQINQQL LNALVEQDDA ERLFRRFRAA GDLPYGAFGE IFWETQCQEM QQLADRVIAC RQPGQSMEID LACNGVQITG WLPQVQPDGL LRWRPSLLSV AQGMQLWLEH LVYCASGGNG ESRLFLRKDG EWRFPPLAAE QALHYLSQLI EGYREGMSAP LLVLPESGGA WLKTCYDAQN DAMLDDDSTL QKARTKFLQA YEGNMMVRGE GDDIWYQRLW RQLTPETMEA IVEQSQRFLL PLFRFNQS . It is sometimes possible for the material contained within the vial of "Exodeoxyribonuclease V gamma chain (recC), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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