Testing Data
Rabbit CD156b (IN) Polyclonal Antibody | anti-CD156b (IN) antibody
Rabbit anti CD156b/ADAM17 Polyclonal antibody
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Synonyms
CD156b (IN); Polyclonal Antibody; Rabbit anti CD156b/ADAM17 Polyclonal antibody; Rabbit anti-CD156b (NT); ADAM17 (A disintegrin and metalloproteinase domain 17); CD156b antigen; TACE (TNF-alpha-converting enzyme); Snake venom-like protease.; anti-CD156b (IN) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody recognizes ~100 kDa of human CD156b. It also reacts with mouse and rat. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-CD156b (IN) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Antigen Preparation
A synthetic peptide corresponding to the human CD156b at extracellular domain. This sequence is identical among human, mouse or rat origins.
Positive Control
Jurkat Cells
Cellular Location
Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-CD156b (IN) antibody
CD156b is a type I transmembrane glycoprotein belong to the ADAM (a disintegrin and metalloprotease domain) family. This single-pass type-1 824 aa transmembrane glycoprotein contains a signal sequence, an extracellular domain (prodomain, catalytic domain, disintegrin-cysteine-rich domain, glycosylation sites), transmembrane and cytoplasmic domain. CD156b is a multi-domain protein that includes a Zn-dependent protease domain in the extracellular portion. It releases the soluble forms of TNF and TGFa from cells. CD156b is identified by its ability to cleave the transmembrane form of TNF between Ala-76 and Val-77 to generate the soluble form. CD156b is therefore a prospective therapeutic target in human cancer. CD156b is expressed on T cells, neutrophils, endothelial cells, monocytes, dendritic cells, macrophages, polymorphonuclear leukocytes and myocytes. And non-lymphoid tissues including heart, placenta, brain, muscle.
References
Gooz,P., et al. ADAM-17 regulates endothelial cell morphology, proliferation, and in vitro angiogenesis Biochem. Biophys. Res. Commun. 380 (1), 33-38 (2009).
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Product Notes
The CD156b (IN) (Catalog #AAA462163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CD156b/ADAM17 Polyclonal antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD156b (IN) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 2-5 ug/ml. Researchers should empirically determine the suitability of the CD156b (IN) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD156b (IN), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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